This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pT7tyrgRNA
catalog :
46761
citations: 3
Reference
Hoff S, Epting D, Falk N, Schroda S, Braun D, Halbritter J, et al. The nucleoside-diphosphate kinase NME3 associates with nephronophthisis proteins and is required for ciliary function during renal development. J Biol Chem. 2018;293:15243-15255 pubmed publisher
Ota S, Kawahara A. Zebrafish: a model vertebrate suitable for the analysis of human genetic disorders. Congenit Anom (Kyoto). 2014;54:8-11 pubmed publisher
Jao L, Wente S, Chen W. Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc Natl Acad Sci U S A. 2013;110:13904-9 pubmed publisher
product information
Catalog Number :
46761
Product Name :
pT7tyrgRNA
article :
doi10.1073/pnas.1308335110
id7188
pubmed_id23918387
bacterial resistance :
Ampicillin
cloning :
backbonepT7-gRNA
backbone_mutation
backbone_origin
backbone_size2542
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
growth notes :
For more information on Chen and Wente Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Chen/ gRNA target sequence CCCCAGAAGTCCTCCAGTCC
origin :
37
pi :
alt_names
tyr
cloning
clone_methodRestriction Enzyme
cloning_site_3BsmBI
cloning_site_5BsmBI
promoter
sequencing_primer_3M13 F
sequencing_primer_5M13 R
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasessandy, zgc:109705
genetyr
id30207
genbank_ids
mutation
nametyr gRNA
shRNA_sequence
size20
species
7955
Danio rerio
tags
resistance markers :
1611
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA