This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
5xGAL4-TATA-luciferase
catalog :
46756
citations: 7
Reference
Sloas D, Tran J, Marzilli A, Ngo J. Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection. Nat Biotechnol. 2023;: pubmed publisher
Keppner A, Correia M, Santambrogio S, Koay T, Maric D, Osterhof C, et al. Androglobin, a chimeric mammalian globin, is required for male fertility. elife. 2022;11: pubmed publisher
Wei Y, Jiang Z, Lu J. USP22 promotes melanoma and BRAF inhibitor resistance via YAP stabilization. Oncol Lett. 2021;21:394 pubmed publisher
Litke A, Samuelson S, Delaney K, Sauve Y, Chow R. Investigating the Pathogenicity of VSX1 Missense Mutations and Their Association With Corneal Disease. Invest Ophthalmol Vis Sci. 2018;59:5824-5835 pubmed publisher
Kofler M, Speight P, Little D, Di Ciano Oliveira C, Szaszi K, Kapus A. Mediated nuclear import and export of TAZ and the underlying molecular requirements. Nat Commun. 2018;9:4966 pubmed publisher
Tague E, Dotson H, Tunney S, Sloas D, Ngo J. Chemogenetic control of gene expression and cell signaling with antiviral drugs. Nat Methods. 2018;15:519-522 pubmed publisher
Sun P, Enslen H, Myung P, Maurer R. Differential activation of CREB by Ca2+/calmodulin-dependent protein kinases type II and type IV involves phosphorylation of a site that negatively regulates activity. Genes Dev. 1994;8:2527-39 pubmed
product information
Catalog Number :
46756
Product Name :
5xGAL4-TATA-luciferase
article :
doi10.1101/gad.8.21.2527
id6616
pubmed_id7958915
bacterial resistance :
Ampicillin
cloning :
backbonepSV232AL-AD5
backbone_mutationdelete SV40 promoter and add 2xSV40 polyadenylation sits, 5xGAL4 binding sites and a minimal TATA promoter upstream of luciferase
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Luciferase
growth notes :
de Wet et al, MCB, 7: 725-737, 1987. Note that there are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These differences should not affect plasmid function.
growth strain :
Luciferase reporter gene for factors with GAL4 DNA binding domains
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterminimal TATA
sequencing_primer_3CGAAAAGTGCCACCTGACGTCT
sequencing_primer_5TGTGTCAGAGGTTTTCACCGT
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameLuciferase
shRNA_sequence
size1729
species
32644
Other
Firefly
tags
plasmid copy :
DR Helinski University of California San Diego
resistance markers :
1631
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA