pQHA-USP7 CS puroR | Addgene 46754 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pQHA-USP7 CS puroR

catalog :

46754

citations: 2

Reference
Biswas K, Philip S, Yadav A, Martin B, Burkett S, Singh V, et al. BRE/BRCC45 regulates CDC25A stability by recruiting USP7 in response to DNA damage. Nat Commun. 2018;9:537 pubmed publisher
Maertens G, El Messaoudi Aubert S, Elderkin S, Hiom K, Peters G. Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation of the INK4a tumour suppressor. EMBO J. 2010;29:2553-65 pubmed publisher

product information

Catalog Number :

46754

Product Name :

pQHA-USP7 CS puroR

article :

doi	10.1038/emboj.2010.129
id	6944
pubmed_id	20601937

bacterial resistance :

Ampicillin

cloning :

backbone

pQCXIP

backbone_mutation

pQHA-puro was created by ligation into the MfeI- and AgeI-digested pQFlag-puro (pQFlag-puro was engineered by ligation in NotI- and BamHI-digested pQCXIP, giving the following MCS: NotI-Kozak-MfeI-Flag-AgeI-XhoI-BamHI).

backbone_origin

Clontech

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

Sequence discrepancies between the full and QC sequences have no functional consequence.

growth strain :

Retroviral vector that expresses catalytically inactive form of HA-tagged human USP7

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	AgeI
promoter	CMV
sequencing_primer_3	pCDH-Rev
sequencing_primer_5	CMV Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1
gene	USP7
id	7874

genbank_ids

mutation

C223S--catalytically inactive

name

USP7

shRNA_sequence

size

species

9606

Homo sapiens