This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABE-Puro-KRas*G12V
catalog :
46746
citations: 13
Reference
Gong Z, Xue L, Vlantis A, van Hasselt C, Chan J, Fang J, et al. Brusatol attenuated proliferation and invasion induced by KRAS in differentiated thyroid cancer through inhibiting Nrf2. J Endocrinol Invest. 2023;: pubmed publisher
Xu C, Gao Q, Wu Z, Lou W, Li X, Wang M, et al. Combined HASPIN and mTOR inhibition is synergistic against KRAS-driven carcinomas. Transl Oncol. 2022;26:101540 pubmed publisher
Alhayyani S, McLeod L, West A, Balic J, Hodges C, Yu L, et al. Oncogenic dependency on STAT3 serine phosphorylation in KRAS mutant lung cancer. Oncogene. 2021;: pubmed publisher
Castellan M, Guarnieri A, Fujimura A, Zanconato F, Battilana G, Panciera T, et al. Single-cell analyses reveal YAP/TAZ as regulators of stemness and cell plasticity in Glioblastoma. Nat Cancer. 2021;2:174-188 pubmed publisher
Ye H, Liu Y, Wu K, Luo H, Cui L. AMPK activation overcomes anti-EGFR antibody resistance induced by KRAS mutation in colorectal cancer. Cell Commun Signal. 2020;18:115 pubmed publisher
Kumazaki M, Shimomura I, Kiyono T, Ochiya T, Yamamoto Y. Cell-type specific tumorigenesis with Ras oncogenes in human lung epithelial cells. Biochem Biophys Res Commun. 2020;525:483-490 pubmed publisher
Ogawa F, Walters M, Shafquat A, O Beirne S, Kaner R, Mezey J, et al. Role of KRAS in regulating normal human airway basal cell differentiation. Respir Res. 2019;20:181 pubmed publisher
Liu H, Sun M, Liu Z, Kong C, Kong W, Ye J, et al. KRAS-enhanced macropinocytosis and reduced FcRn-mediated recycling sensitize pancreatic cancer to albumin-conjugated drugs. J Control Release. 2019;296:40-53 pubmed publisher
Bennett Saidu N, Bretagne M, Mansuet A, Just P, Leroy K, Cerles O, et al. Dimethyl fumarate is highly cytotoxic in KRAS mutated cancer cells but spares non-tumorigenic cells. Oncotarget. 2018;9:9088-9099 pubmed publisher
Mulcahy Levy J, Zahedi S, Griesinger A, Morin A, Davies K, Aisner D, et al. Autophagy inhibition overcomes multiple mechanisms of resistance to BRAF inhibition in brain tumors. elife. 2017;6: pubmed publisher
Weaver R, Limzerwala J, Naylor R, Jeganathan K, Baker D, van Deursen J. BubR1 alterations that reinforce mitotic surveillance act against aneuploidy and cancer. elife. 2016;5: pubmed publisher
Wiersma V, de Bruyn M, Wei Y, van Ginkel R, Hirashima M, Niki T, et al. The epithelial polarity regulator LGALS9/galectin-9 induces fatal frustrated autophagy in KRAS mutant colon carcinoma that depends on elevated basal autophagic flux. Autophagy. 2015;11:1373-88 pubmed publisher
Lampson B, Pershing N, Prinz J, Lacsina J, Marzluff W, Nicchitta C, et al. Rare codons regulate KRas oncogenesis. Curr Biol. 2013;23:70-5 pubmed publisher
product information
Catalog Number :
46746
Product Name :
pBABE-Puro-KRas*G12V
article :
doi10.1016/j.cub.2012.11.031
id6943
pubmed_id23246410
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-Puro
backbone_mutation
backbone_origin
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth strain :
Expresses human oncogenic KRas protein from chimeric cDNA sequence derived primarily from HRAS codons with a KRAS 3' tail. Results in higher than normal exogenous expression of KRas protein.
inserts :
XL10 Gold
origin :
37
pi :
alt_names
V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3pBABE 3'
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
mutationcarries the oncogenic G12V mutation and cDNA is encoded by a chimeric mixture of HRAS and KRAS codons that encode KRas protein
nameKRAS*G12V
shRNA_sequence
size591
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tagFLAG
resistance markers :
95
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA