This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABE-Puro-KRas*
catalog :
46745
citations: 2
Reference
Ye H, Liu Y, Wu K, Luo H, Cui L. AMPK activation overcomes anti-EGFR antibody resistance induced by KRAS mutation in colorectal cancer. Cell Commun Signal. 2020;18:115 pubmed publisher
Lampson B, Pershing N, Prinz J, Lacsina J, Marzluff W, Nicchitta C, et al. Rare codons regulate KRas oncogenesis. Curr Biol. 2013;23:70-5 pubmed publisher
product information
Catalog Number :
46745
Product Name :
pBABE-Puro-KRas*
article :
doi10.1016/j.cub.2012.11.031
id6943
pubmed_id23246410
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-Puro
backbone_mutation
backbone_origin
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth strain :
Expresses human KRas protein from chimeric cDNA sequence derived primarily from HRAS codons with a KRAS 3' tail. Results in higher than normal exogenous expression of KRas protein.
inserts :
XL10 Gold
origin :
37
pi :
alt_names
V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3pBABE 3'
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
mutationcDNA is encoded by a chimeric mixture of HRAS and KRAS codons that encode KRas protein
nameKRAS*
shRNA_sequence
size
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tagFLAG
resistance markers :
95
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA