This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.2 mir-1-1 reporter mir16-1.1
catalog :
46680
citations: 1
Reference
Auyeung V, Ulitsky I, McGeary S, Bartel D. Beyond secondary structure: primary-sequence determinants license pri-miRNA hairpins for processing. Cell. 2013;152:844-58 pubmed publisher
product information
Catalog Number :
46680
Product Name :
pcDNA3.2 mir-1-1 reporter mir16-1.1
article :
doi10.1016/j.cell.2013.01.031
id6423
pubmed_id23415231
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.2 V5-DEST mir-1-1 reporter
backbone_mutation
backbone_originBartel lab (Addgene plasmid# 46646)
backbone_size8038
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
RNAi
growth strain :
Expresses a mutant version of the wild-type miR-16-1 in mammalian cells
origin :
37
pi :
alt_names
mutant version of mir16-1 pri-mRNA
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3TK-pA-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIRN16-1, miRNA16-1, mir-16-1
geneMIR16-1
id406950
genbank_ids
mutationsee sequence and publication
namemir16-1.1
shRNA_sequence
size
species
9606
Homo sapiens
100
Synthetic
tags
locationC terminal on insert
taghsa-mir-1-1
locationC terminal on backbone
tagV5
resistance markers :
21
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA