pMXs-hM O-IP | Addgene 46645 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMXs-hM O-IP

catalog :

46645

citations: 2

Reference
Kogut I, McCarthy S, Pavlova M, Astling D, Chen X, Jakimenko A, et al. High-efficiency RNA-based reprogramming of human primary fibroblasts. Nat Commun. 2018;9:745 pubmed publisher
Hirai H, Tani T, Katoku Kikyo N, Kellner S, Karian P, Firpo M, et al. Radical acceleration of nuclear reprogramming by chromatin remodeling with the transactivation domain of MyoD. Stem Cells. 2011;29:1349-61 pubmed publisher

product information

Catalog Number :

46645

Product Name :

pMXs-hM O-IP

article :

doi	10.1002/stem.684
id	6941
pubmed_id	21732495

bacterial resistance :

Ampicillin

cloning :

backbone

pMXs-IP

backbone_mutation

backbone_origin

Toshio Kitamura lab, University of Tokyo (PMID: 14585362)

backbone_size

5847

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

origin :

pi :

alt_names

human Oct3/4

Pou5f1

octamer-binding transcription factor 4

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	EcoRI
promoter
sequencing_primer_3	pCDH-rev (5'-GCATTCCTTTGGCGAGAG-3'); puro-variant-R (5'-gtgggcttgtactcggtcat-3')
sequencing_primer_5	pBMN 5' (5'-GCTTGGATACACGCCGC-3'); pMX-S1811 (5'-GACGGCATCGCAGCTTGGATACAC-3')
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
gene	POU5F1
id	5460

genbank_ids

NM_002701.4

NP_002692.2

mutation

contains a fragment of human MyoD (aa1-62) fused to the N-terminus of full length human Oct3/4

name

hM O

shRNA_sequence

size

1286

species

9606

Homo sapiens