This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCOCK CMVwt
catalog :
46559
citations: 1
Reference
Ferreira J, Peacock R, Lawhorn I, Wang C. Modulating ectopic gene expression levels by using retroviral vectors equipped with synthetic promoters. Syst Synth Biol. 2011;5:131-8 pubmed publisher
product information
Catalog Number :
46559
Product Name :
pCOCK CMVwt
article :
doi10.1007/s11693-011-9089-0
id6892
pubmed_id23205156
bacterial resistance :
Ampicillin
cloning :
backbonepEF5-FRT-V5-DEST
backbone_mutation1. EF1alpha promoter removed (so attR Gateway sites can be used to clone in synthetic promoters) 2. IVS-EGFP cassette inserted between attR Gateway cassette and BGH polyA 3. Synthetic promoters flanked by attL in pDONR221 (Life Technologies) vectors were used to recombine promoters into the vector resulting from modifications 1 and 2 above.
backbone_originLife Technologies
backbone_size7528
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Synthetic Biology
growth strain :
Mammalian expression plasmid with EGFP expressed from CMV wild-type promoter.
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3T3
sequencing_primer_5CMV 5' end primer
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
# K03104.1 (bases 156 to 744)
mutationOur version has a G at base 724 (reference has C)
nameCMV wt promoter
shRNA_sequence
size589
species
32644
Other
Cytomegalovirus viral promoter
tags
resistance markers :
1699
tags :
High Copy
terms :
Hygromycin
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA