pGEX Grb2-SH2 | Addgene 46440 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGEX Grb2-SH2

catalog :

46440

citations: 3

Reference
Luo L, Tong S, Wall A, Khromykh T, Sweet M, Stow J. Development of SH2 probes and pull-down assays to detect pathogen-induced, site-specific tyrosine phosphorylation of the TLR adaptor SCIMP. Immunol Cell Biol. 2017;95:564-570 pubmed publisher
Keppel T, Sarpong K, Murray E, Monsey J, Zhu J, Bose R. Biophysical Evidence for Intrinsic Disorder in the C-terminal Tails of the Epidermal Growth Factor Receptor (EGFR) and HER3 Receptor Tyrosine Kinases. J Biol Chem. 2017;292:597-610 pubmed publisher
Machida K, Thompson C, Dierck K, Jablonowski K, Kärkkäinen S, Liu B, et al. High-throughput phosphotyrosine profiling using SH2 domains. Mol Cell. 2007;26:899-915 pubmed

product information

Catalog Number :

46440

Product Name :

pGEX Grb2-SH2

article :

doi	10.1016/j.molcel.2007.05.031
id	6931
pubmed_id	17588523

bacterial resistance :

Ampicillin

cloning :

backbone

pGEX2T

backbone_mutation

backbone_origin

GE Healthcare Biosciences

backbone_size

4900

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

The molecular weight of the GST fusion protein is approximately 36.6 kDa

origin :

pi :

alt_names

GRB2

ASH; Grb3-3; MST084; MSTP084; EGFRBP-GRB2; GRB2

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter	Tac
sequencing_primer_3	pGEX-3
sequencing_primer_5	pGEX-5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2
gene	GRB2
id	2885

genbank_ids

mutation

contains amino acids 58-159

name

Grb2

shRNA_sequence

size

319

species

9606

Homo sapiens