This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1 EWS-myc-HIS
catalog :
46386
citations: 2
Reference
Caropreso V, Darvishi E, Turbyville T, Ratnayake R, Grohar P, McMahon J, et al. Englerin A Inhibits EWS-FLI1 DNA Binding in Ewing Sarcoma Cells. J Biol Chem. 2016;291:10058-66 pubmed publisher
Pahlich S, Zakaryan R, Gehring H. Identification of proteins interacting with protein arginine methyltransferase 8: the Ewing sarcoma (EWS) protein binds independent of its methylation state. Proteins. 2008;72:1125-37 pubmed publisher
product information
Catalog Number :
46386
Product Name :
pcDNA3.1 EWS-myc-HIS
article :
doi10.1002/prot.22004
id6916
pubmed_id18320585
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(-)B/myc-His
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
EWS cDNA was amplified by PCR using XhoI-NotI-forward and EcoRI-reverse primers 5-TCTCTCGAGCG GCCGCGCCACCATGGCGTCCACGGATTACAGTACC-3 5-CCGAATTCGTAGGGCCGATCTCTGCGCTCCTG-3, respectively.
growth strain :
expresses myc, HIS tagged EWSR1 in mammalian cells
origin :
37
pi :
alt_names
EWS
EWS RNA-binding protein 1 isoform CRA_e
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3BGHrev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesEWS, EWS-FLI1, bK984G1.4
geneEWSR1
id2130
genbank_ids
mutation
nameEWSR1
shRNA_sequence
size2600
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagmyc
locationC terminal on backbone
tagHis
resistance markers :
1633
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA