This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1 His-GFP-EWSR1-myc-His
catalog :
46385
citations: 2
Reference
Li Y, Zhu L, Ko C, Yang J, Wang H, Manyam G, et al. TRAF3-EWSR1 signaling axis acts as a checkpoint on germinal center responses. J Exp Med. 2023;220: pubmed publisher
Pahlich S, Zakaryan R, Gehring H. Identification of proteins interacting with protein arginine methyltransferase 8: the Ewing sarcoma (EWS) protein binds independent of its methylation state. Proteins. 2008;72:1125-37 pubmed publisher
product information
Catalog Number :
46385
Product Name :
pcDNA3.1 His-GFP-EWSR1-myc-His
article :
doi10.1002/prot.22004
id6916
pubmed_id18320585
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(-)B/myc-His
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The EWS-myc-His sequence was amplified by PCR using a XhoI-NotI-forward primer, a SacII His-reverse primer, and pcDNA3.1(-)B-EWS-myc-His (Addgene plasmid 46386) expression vector as a template. The resulting fragment was digested with XhoI and SacII restriction enzymes, and incorporated into the pEGFP-N2 plasmid (Clontech). The His-GFP fragment from pEGFP-N2-EWS-myc-His was amplified using a NheI-His forward and NotI-GFP-reverse primer and cloned into the pcDNA3.1(-)B-EWS-myc-His plasmid to create the final His-EGFP-EWS-myc-HIS plasmid. This plasmid contains the Y583F point mutation which was specially introduced to disrupt NLS function. This construct can serve as a negative control for nuclear localization, as well as for tyrosine phosphorylation.
growth strain :
expresses EGFP tagged EWSR1 in mammalian cells
origin :
37
pi :
alt_names
EWS
EWS RNA-binding protein 1 isoform CRA_e
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5NheI
promoterCMV
sequencing_primer_3BGHrev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesEWS, EWS-FLI1, bK984G1.4
geneEWSR1
id2130
genbank_ids
mutation
nameEWSR1
shRNA_sequence
size2600
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHis
locationN terminal on insert
tagEGFP
locationC terminal on backbone
tagmyc
locationC terminal on backbone
tagHis
resistance markers :
1633
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA