This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-6p-2-EWSR1
catalog :
46384
citations: 2
Reference
Kedage V, Selvaraj N, Nicholas T, Budka J, Plotnik J, Jerde T, et al. An Interaction with Ewing's Sarcoma Breakpoint Protein EWS Defines a Specific Oncogenic Mechanism of ETS Factors Rearranged in Prostate Cancer. Cell Rep. 2016;17:1289-1301 pubmed publisher
Pahlich S, Bschir K, Chiavi C, Belyanskaya L, Gehring H. Different methylation characteristics of protein arginine methyltransferase 1 and 3 toward the Ewing Sarcoma protein and a peptide. Proteins. 2005;61:164-75 pubmed
product information
Catalog Number :
46384
Product Name :
pGEX-6p-2-EWSR1
article :
doi10.1002/prot.20579
id6915
pubmed_id16044463
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-6p-2
backbone_mutation
backbone_originAmersham
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
and CTGGTAGTCAATGCAGCTCGAGGGGGTCTCTGCATCTAGTAGG.
ATCTGGAATTCAAATGGCGTCCACGGATTACAGTACCTA
TAGCCAAGCTGCAGC
The EWS cDNA23 was PCR-amplified using primers
origin :
37
pi :
alt_names
EWS
EWS RNA-binding protein 1 isoform CRA_e
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3pGEX3'
sequencing_primer_5pGEX5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesEWS, EWS-FLI1, bK984G1.4
geneEWSR1
id2130
genbank_ids
mutation
nameEWSR1
shRNA_sequence
size2600
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
resistance markers :
1633
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA