This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBS-Hsp70-Cas9
catalog :
46294
citations: 24
Reference
Chennuri P, Zapletal J, Monfardini R, Ndeffo Mbah M, Adelman Z, Myles K. Repeat mediated excision of gene drive elements for restoring wild-type populations. bioRxiv. 2023;: pubmed publisher
Novas R, Basika T, Williamson M, Fresia P, Menchaca A, Scott M. Identification and functional analysis of Cochliomyia hominivorax U6 gene promoters. Insect Mol Biol. 2023;32:716-724 pubmed publisher
Hausjell C, Klausberger M, Ernst W, Grabherr R. Evaluation of an inducible knockout system in insect cells based on co-infection and CRISPR/Cas9. PLoS ONE. 2023;18:e0289178 pubmed publisher
Hamamoto K, Umemura Y, Makino S, Fukaya T. Dynamic interplay between non-coding enhancer transcription and gene activity in development. Nat Commun. 2023;14:826 pubmed publisher
Reid W, Williams A, Sanchez Vargas I, Lin J, Juncu R, Olson K, et al. Assessing single-locus CRISPR/Cas9-based gene drive variants in the mosquito Aedes aegypti via single-generation crosses and modeling. G3 (Bethesda). 2022;12: pubmed publisher
Baumgartner L, Handler D, Platzer S, Yu C, Duchek P, Brennecke J. The Drosophila ZAD zinc finger protein Kipferl guides Rhino to piRNA clusters. elife. 2022;11: pubmed publisher
Bishop A, López Del Amo V, Okamoto E, Bodai Z, Komor A, Gantz V. Double-tap gene drive uses iterative genome targeting to help overcome resistance alleles. Nat Commun. 2022;13:2595 pubmed publisher
Bai Y, Caussinus E, Léo S, Bosshardt F, Myachina F, Rot G, et al. A cis-regulatory element promoting increased transcription at low temperature in cultured ectothermic Drosophila cells. BMC Genomics. 2021;22:771 pubmed publisher
Miyata Y, Fuse H, Tokumoto S, Hiki Y, Deviatiiarov R, Yoshida Y, et al. Cas9-mediated genome editing reveals a significant contribution of calcium signaling pathways to anhydrobiosis in Pv11 cells. Sci Rep. 2021;11:19698 pubmed publisher
Yan Y, Kobayashi Y, Huang C, Liu B, Qian W, Wan F, et al. Highly Efficient Temperature Inducible CRISPR-Cas9 Gene Targeting in Drosophila suzukii. Int J Mol Sci. 2021;22: pubmed publisher
Li Z, Marcel N, Devkota S, Auradkar A, Hedrick S, Gantz V, et al. CopyCatchers are versatile active genetic elements that detect and quantify inter-homolog somatic gene conversion. Nat Commun. 2021;12:2625 pubmed publisher
Johnstun J, Shankar V, Mokashi S, Sunkara L, Ihearahu U, Lyman R, et al. Functional Diversification, Redundancy and Epistasis among Paralogs of the Drosophila melanogaster Obp50a-d Gene Cluster. Mol Biol Evol. 2021;: pubmed publisher
Trivedi D, Cm V, Bisht K, Janardan V, Pandit A, Basak B, et al. A genome engineering resource to uncover principles of cellular organization and tissue architecture by lipid signaling. elife. 2020;9: pubmed publisher
Onishi R, Sato K, Murano K, Negishi L, Siomi H, Siomi M. Piwi suppresses transcription of Brahma-dependent transposons via Maelstrom in ovarian somatic cells. Sci Adv. 2020;6: pubmed publisher
Cohen L, Lindsay S, Xu Y, Lin S, Wasserman S. The Daisho Peptides Mediate Drosophila Defense Against a Subset of Filamentous Fungi. Front Immunol. 2020;11:9 pubmed publisher
Lin S, Fulzele A, Cohen L, Bennett E, Wasserman S. Bombardier Enables Delivery of Short-Form Bomanins in the Drosophila Toll Response. Front Immunol. 2019;10:3040 pubmed publisher
Ahmed H, Hildebrand L, Wimmer E. Improvement and use of CRISPR/Cas9 to engineer a sperm-marking strain for the invasive fruit pest Drosophila suzukii. BMC Biotechnol. 2019;19:85 pubmed publisher
Golas M, Jayaprakash S, Le L, Zhao Z, Heras Huertas V, Jensen I, et al. Modulating the Expression Strength of the Baculovirus/Insect Cell Expression System: A Toolbox Applied to the Human Tumor Suppressor SMARCB1/SNF5. Mol Biotechnol. 2018;60:820-832 pubmed publisher
Lindsay S, Lin S, Wasserman S. Short-Form Bomanins Mediate Humoral Immunity in Drosophila. J Innate Immun. 2018;10:306-314 pubmed publisher
Ishizu H, Sumiyoshi T, Siomi M. Use of the CRISPR-Cas9 system for genome editing in cultured Drosophila ovarian somatic cells. Methods. 2017;126:186-192 pubmed publisher
Lamb A, Walker E, Wittkopp P. Tools and strategies for scarless allele replacement in Drosophila using CRISPR/Cas9. Fly (Austin). 2017;11:53-64 pubmed publisher
Sumiyoshi T, Sato K, Yamamoto H, Iwasaki Y, Siomi H, Siomi M. Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells. Genes Dev. 2016;30:1617-22 pubmed publisher
Brinkman E, Chen T, Amendola M, van Steensel B. Easy quantitative assessment of genome editing by sequence trace decomposition. Nucleic Acids Res. 2014;42:e168 pubmed publisher
Gratz S, Ukken F, Rubinstein C, Thiede G, Donohue L, Cummings A, et al. Highly specific and efficient CRISPR/Cas9-catalyzed homology-directed repair in Drosophila. Genetics. 2014;196:961-71 pubmed publisher
product information
Catalog Number :
46294
Product Name :
pBS-Hsp70-Cas9
article :
doi10.1534/genetics.113.160713
id28224911
pubmed_id24478335
bacterial resistance :
Ampicillin
cloning :
backbonepBluescript-KS(+)
backbone_mutation
backbone_originStratagene
backbone_size2958
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
CRISPR
growth notes :
This plasmid was created by subcloning the Hsp70-Cas9 containing NotI fragment from Addgene plasmid 45945 in NotI digested pBluescript-KS(+). For more information on FlyCRISPR Plasmids please refer to: http://www.addgene.org/crispr/OConnor-Giles/ Plasmid 51019: pDsRed-attP (www.addgene.org/51019) can be for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site.
growth strain :
A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.
origin :
37
pi :
alt_names
SpCas9
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NotI
promoterHsp70
sequencing_primer_3T3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationSilent change from C to A at bp 834 of insert
namecodon optimized Cas9
shRNA_sequence
size
species
tags
locationN terminal on insert
tag3x Flag
plasmid copy :
Cas9 insert from Feng Zhang, Broad Institute, MIT
resistance markers :
1594
1592
1593
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA