This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GgVcl 1-1066 FRET tail probe 2
catalog :
46282
citations: 1
Reference
Chen H, Choudhury D, Craig S. Coincidence of actin filaments and talin is required to activate vinculin. J Biol Chem. 2006;281:40389-98 pubmed
product information
Catalog Number :
46282
Product Name :
GgVcl 1-1066 FRET tail probe 2
article :
doi10.1074/jbc.M607324200
id6891
pubmed_id17074767
bacterial resistance :
Kanamycin
cloning :
backbonepECFP-N3
backbone_mutation
backbone_originClonetech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Similar to FRET probe 1 (Addgene plasmid 46276) except ECFP changed to monomeric Citrine.
origin :
37
pi :
alt_names
Vinculin
Vcl
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3?
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesVINC1
geneVCL
id396422
genbank_ids
mutationmCitrine (and linker) inserted after aa883
nameVcl 1-1066 + mCitrine
shRNA_sequence
size
species
9031
Gallus gallus
tags
locationC terminal on backbone
tagECFP
resistance markers :
1616
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA