pEYFPC1-ECFP/GgVcl 1-419 control 1 FRET | Addgene 46277 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEYFPC1-ECFP/GgVcl 1-419 control 1 FRET

catalog :

46277

citations: 1

Reference
Chen H, Cohen D, Choudhury D, Kioka N, Craig S. Spatial distribution and functional significance of activated vinculin in living cells. J Cell Biol. 2005;169:459-70 pubmed

product information

Catalog Number :

46277

Product Name :

pEYFPC1-ECFP/GgVcl 1-419 control 1 FRET

article :

doi	10.1083/jcb.200410100
id	6881
pubmed_id	15883197

bacterial resistance :

Kanamycin

cloning :

backbone

pEYFP-C1

backbone_mutation

backbone_origin

Clonetech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

To make the control FRET probe pEYFP-C1/CFPV1-400, a HindIII site was engineered before the ATG site of pET15b/CFP-V1-851 and a KpnI site after codon 400 by PCR amplification with 5-CAAGCTTCGatggtgagcaagggc-3 and 5-GGTACCTCAtgcaactttccttgc-3. The PCR product was introduced into TOPO pCRII and sequenced. The HindIII KpnI fragment of CFP-vinculin1-400 was subcloned into pEYFP-C1 to generate the control plasmid pEYFP-CFPV1-400. Tandem EYFP-ECFP- hooked to portion of vinculin that localizes to FA; maximum FRET.

origin :

pi :

alt_names

Vinculin

Vcl

cloning

clone_method	Restriction Enzyme
cloning_site_3	KpnI
cloning_site_5	HindIII
promoter	CMV
sequencing_primer_3	SV40pA-R
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	VINC1
gene	VCL
id	396422

genbank_ids

mutation

aa 1-419

name

ECFP + Vcl 1-419

shRNA_sequence

size

species

9031

Gallus gallus