GgVcl 1-1066 FRET tail probe 1 | Addgene 46276 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

GgVcl 1-1066 FRET tail probe 1

catalog :

46276

citations: 1

Reference
Chen H, Cohen D, Choudhury D, Kioka N, Craig S. Spatial distribution and functional significance of activated vinculin in living cells. J Cell Biol. 2005;169:459-70 pubmed

product information

Catalog Number :

46276

Product Name :

GgVcl 1-1066 FRET tail probe 1

article :

doi	10.1083/jcb.200410100
id	6881
pubmed_id	15883197

bacterial resistance :

Kanamycin

cloning :

backbone

pECFP-N3

backbone_mutation

pECFP-N3 vector was constructed as an intermediate vector for generating tail probe. ECFP was PCR amplified with 5-GGTACCatggtgagcaagggc-3 and 5-GCGGCCGCTttacttgtacagctc-3 to generate 5' KpnI and 3' NotI. The product was subcloned into TOPO pCRII and sequenced. The KpnI and NotI fragment of pCRII/ECFP was used to replace the EGFP fragment of pEGFP-N3 to generate pECFP-N3.

backbone_origin

Craig lab

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

A 9-bp fragment encoding a NotI site was introduced by mutagenesis into pEGFP-C1/vinculin cDNA (chicken) immediately after the codon for aa 883 (Coutu and Craig, 1988) using the Quick Change kit (Stratagene). The cDNA of EYFP (CLONTECH Laboratories, Inc.) minus the stop codon and flanked by NotI was inserted into NotI-digested vinculin.

origin :

pi :

alt_names

Vinculin

Vcl

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3	?
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	VINC1
gene	VCL
id	396422

genbank_ids

mutation

addition of 9 bp linker and EYFP at aa 883

name

Vcl 1-1066 + EYFP

shRNA_sequence

size

species

9031

Gallus gallus