pEGFPC1/GgVcl 1-258 A50I mutation | Addgene 46271 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEGFPC1/GgVcl 1-258 A50I mutation

catalog :

46271

citations: 2

Reference
Elosegui Artola A, Oria R, Chen Y, Kosmalska A, Pérez González C, Castro N, et al. Mechanical regulation of a molecular clutch defines force transmission and transduction in response to matrix rigidity. Nat Cell Biol. 2016;18:540-8 pubmed publisher
Cohen D, Kutscher B, Chen H, Murphy D, Craig S. A conformational switch in vinculin drives formation and dynamics of a talin-vinculin complex at focal adhesions. J Biol Chem. 2006;281:16006-15 pubmed

product information

Catalog Number :

46271

Product Name :

pEGFPC1/GgVcl 1-258 A50I mutation

article :

doi	10.1074/jbc.M600738200
id	6879
pubmed_id	16608855

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

vector modified to contain unique Nde, Xho sites.

backbone_origin

Clonetech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

A50I mutation introduced into EGFP/V1-258 (Addgene plasmid 46270) by QCPCR.

origin :

pi :

alt_names

Vinculin

Vcl

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NdeI
promoter	CMV
sequencing_primer_3	SV40 pA R
sequencing_primer_5	EGFP-C
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	VINC1
gene	VCL
id	396422

genbank_ids

NM_205441.1

mutation

1-258 (VD1 domain) A50I mutation

name

Vcl 1-258 (aka VD1) A50I mutation

shRNA_sequence

size

species

9031

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