pEGFPC1/GgVcl 1-258 (aka VD1) | Addgene 46270 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEGFPC1/GgVcl 1-258 (aka VD1)

catalog :

46270

citations: 4

Reference
Bennett M, Cantini M, Reboud J, Cooper J, Roca Cusachs P, Salmerón Sánchez M. Molecular clutch drives cell response to surface viscosity. Proc Natl Acad Sci U S A. 2018;115:1192-1197 pubmed publisher
Bertocchi C, Wang Y, Ravasio A, Hara Y, Wu Y, Sailov T, et al. Nanoscale architecture of cadherin-based cell adhesions. Nat Cell Biol. 2017;19:28-37 pubmed publisher
Elosegui Artola A, Oria R, Chen Y, Kosmalska A, Pérez González C, Castro N, et al. Mechanical regulation of a molecular clutch defines force transmission and transduction in response to matrix rigidity. Nat Cell Biol. 2016;18:540-8 pubmed publisher
Cohen D, Kutscher B, Chen H, Murphy D, Craig S. A conformational switch in vinculin drives formation and dynamics of a talin-vinculin complex at focal adhesions. J Biol Chem. 2006;281:16006-15 pubmed

product information

Catalog Number :

46270

Product Name :

pEGFPC1/GgVcl 1-258 (aka VD1)

article :

doi	10.1074/jbc.M600738200
id	6879
pubmed_id	16608855

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

vector modified to contain unique Nde, Xho sites.

backbone_origin

Clonetech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Nde-Xho fragment from pET15b/Vh1-258 (Addgene plasmid 46173) was subcloned into EGFP/V1-1066 (Addgene plasmid 46265) vector modified to contain unique Nde, Xho sites. Modifications were made by QuikChange PCR to facilitate cloning of cDNAs previously in the pET15b vector. The NdeI site present in the cytomegalovirus promoter region was removed (mutating adenine 234 to thymine), and a new NdeI site was introduced into the multiple cloning site immediately before the vinculin start codon. Additionally, the 5-XhoI site was removed (mutating cytosine 1345 to adenine), and a 3-XhoI site was introduced 3' to the vinculin cDNA in lieu of a PstI site.

origin :

pi :

alt_names

Vinculin

Vcl

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NdeI
promoter	CMV
sequencing_primer_3	SV40pA-R
sequencing_primer_5	EGFP-C
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	VINC1
gene	VCL
id	396422

genbank_ids

NM_205441.1

mutation

1-258 (VD1 domain)

name

Vcl 1-258 (aka VD1)

shRNA_sequence

size

774

species

9031

Gallus gallus