This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFPC1/GgVcl 1-851 A50I mutant
catalog :
46269
citations: 3
Reference
Lacroix A, Lynch A, Berginski M, Hoffman B. Tunable molecular tension sensors reveal extension-based control of vinculin loading. elife. 2018;7: pubmed publisher
Rothenberg K, Scott D, Christoforou N, Hoffman B. Vinculin Force-Sensitive Dynamics at Focal Adhesions Enable Effective Directed Cell Migration. Biophys J. 2018;114:1680-1694 pubmed publisher
Cohen D, Kutscher B, Chen H, Murphy D, Craig S. A conformational switch in vinculin drives formation and dynamics of a talin-vinculin complex at focal adhesions. J Biol Chem. 2006;281:16006-15 pubmed
product information
Catalog Number :
46269
Product Name :
pEGFPC1/GgVcl 1-851 A50I mutant
article :
doi10.1074/jbc.M600738200
id6879
pubmed_id16608855
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutationvector modified to contain unique Nde, Xho sites.
backbone_originClonetech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
A50I mutation introduced into EGFP/V1-851 (Addgene plasmid 46268) by QCPCR mutation.
origin :
37
pi :
alt_names
Vinculin
Vcl
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoterCMV
sequencing_primer_3SV40 pA R
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesVINC1
geneVCL
id396422
genbank_ids
NM_205441.1
mutation1-851 (Vh) head domain, A50I mutation
nameVcl 1-851 A50I mutation
shRNA_sequence
size2550
species
9031
Gallus gallus
tags
locationN terminal on backbone
tagEGFP
resistance markers :
1616
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA