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product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFPC1/GgVcl 1-851 (Vh)
catalog :
46268
citations: 2
| Reference |
|---|
Cohen D, Kutscher B, Chen H, Murphy D, Craig S. A conformational switch in vinculin drives formation and dynamics of a talin-vinculin complex at focal adhesions. J Biol Chem. 2006;281:16006-15 pubmed
|
product information
Catalog Number :
46268
Product Name :
pEGFPC1/GgVcl 1-851 (Vh)
article :
| doi | 10.1074/jbc.M600738200 |
| id | 6879 |
| pubmed_id | 16608855 |
bacterial resistance :
Kanamycin
cloning :
| backbone | pEGFP-C1 | |
| backbone_mutation | vector modified to contain unique Nde, Xho sites. | |
| backbone_origin | Clonetech | |
| backbone_size | 4700 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
Nde-Xho fragment from pET15b/Vh1-851 (Addgene plasmid 46172) was subcloned into EGFP/V1-1066 (Addgene plasmid 46265) vector modified to contain unique Nde, Xho sites. Modifications were made by QuikChange PCR to facilitate cloning of cDNAs previously in the pET15b vector. The NdeI site present in the cytomegalovirus promoter region was removed (mutating adenine 234 to thymine), and a new NdeI site was introduced into the multiple cloning site immediately before the vinculin start codon. Additionally, the 5-XhoI site was removed (mutating cytosine 1345 to adenine), and a 3-XhoI site was introduced 3' to the vinculin cDNA in lieu of a PstI site.
origin :
37
pi :
|
resistance markers :
| 1616 |
tags :
Unknown
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments