This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFPC1/GgVcl 1-1066 A50I K1306A, R1308A mutant
catalog :
46267
product information
Catalog Number :
46267
Product Name :
pEGFPC1/GgVcl 1-1066 A50I K1306A, R1308A mutant
article :
doi
id6882
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClonetech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Chicken vinculin, full length(EcoRI fragment of p1005 subcloned into EcoRI-cut pEGFP-C1); A50I mutation (alanine 50 to isoleucine) introduced by QCPCR. This mutant binds poorly (undetectably to talin in pull down assays; sensitivity of assay Kd= 10-20 uM).
origin :
37
pi :
alt_names
Vinculin
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesVINC1
geneVCL
id396422
genbank_ids
NM_205441.1
mutationK1306A, R1308A, A50I mutation; talin binding deficient
nameVcl
shRNA_sequence
size3259
species
9031
Gallus gallus
tags
locationN terminal on backbone
tagEGFP
resistance markers :
1616
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA