pEGFPC1/GgVcl 1-1066 T12 mutant | Addgene 46266 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEGFPC1/GgVcl 1-1066 T12 mutant

catalog :

46266

citations: 2

Reference
Bertocchi C, Wang Y, Ravasio A, Hara Y, Wu Y, Sailov T, et al. Nanoscale architecture of cadherin-based cell adhesions. Nat Cell Biol. 2017;19:28-37 pubmed publisher
Cohen D, Chen H, Johnson R, Choudhury B, Craig S. Two distinct head-tail interfaces cooperate to suppress activation of vinculin by talin. J Biol Chem. 2005;280:17109-17 pubmed

product information

Catalog Number :

46266

Product Name :

pEGFPC1/GgVcl 1-1066 T12 mutant

article :

doi	10.1074/jbc.M414704200
id	6878
pubmed_id	15728584

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

backbone_origin

Clonetech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

T12 mutation introduced by QuikChange PCR to Addgene plasmid 46265. Mutant inhibits vinculin autoinhibition by ~ 100 fold, vinculin is partially activated and will bind talin, but not actin (unless both talin and actin are present at same time).

origin :

pi :

alt_names

Vinculin

Vcl

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3	SV40pA-R
sequencing_primer_5	EGFP-C
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	VINC1
gene	VCL
id	396422

genbank_ids

NM_205441.1

mutation

T12 mutant (D974;K975;R976; R978A) autoinhibition decreased by 100x

name

Vcl T12 mutant

shRNA_sequence

size

3259

species

9031

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