This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFPC1/GgVcl 1-1066
catalog :
46265
citations: 1
Reference
Cohen D, Chen H, Johnson R, Choudhury B, Craig S. Two distinct head-tail interfaces cooperate to suppress activation of vinculin by talin. J Biol Chem. 2005;280:17109-17 pubmed
product information
Catalog Number :
46265
Product Name :
pEGFPC1/GgVcl 1-1066
article :
doi10.1074/jbc.M414704200
id6878
pubmed_id15728584
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClonetech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
full length chicken vinculin (EcoRI fragment of p1005 subcloned into EcoRI-cut pEGFP-C1). ATG at bp 1390-1392. TAA at bp 4588-90. EGFP at N-terminus.
origin :
37
pi :
alt_names
Vinculin
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3SV40 pA R
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesVINC1
geneVCL
id396422
genbank_ids
NM_205441.1
mutation
nameVcl
shRNA_sequence
size3259
species
9031
Gallus gallus
tags
locationN terminal on backbone
tagEGFP
resistance markers :
1616
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA