pET15b/EYFP-GgVcl 1-258 | Addgene 46263 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET15b/EYFP-GgVcl 1-258

catalog :

46263

citations: 1

Reference
Cohen D, Chen H, Johnson R, Choudhury B, Craig S. Two distinct head-tail interfaces cooperate to suppress activation of vinculin by talin. J Biol Chem. 2005;280:17109-17 pubmed

product information

Catalog Number :

46263

Product Name :

pET15b/EYFP-GgVcl 1-258

article :

doi	10.1074/jbc.M414704200
id	6878
pubmed_id	15728584

bacterial resistance :

Ampicillin

cloning :

backbone

pET-15b

backbone_mutation

backbone_origin

EMD

backbone_size

5700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

EYFP was PCR amplified to introduce NdeI site at both 5' and 3' ends. The NdeI flanked EYFP was inserted into NdeI site of pET15b/Vh1-258 (Addgene plasmid 46173), where V1-258 was cloned between NdeI and XhoI. Plasmid contains a 12-residue spacer between the EYFP and vinculin domain.

origin :

pi :

alt_names

Vinculin

cloning

clone_method	Restriction Enzyme
cloning_site_3	Xho1
cloning_site_5	Nde1
promoter	T7
sequencing_primer_3	T7 terminal
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	VINC1
gene	VCL
id	396422

genbank_ids

NM_205441.1

mutation

aa 1-258 (VD1)

name

Vcl 1-258

shRNA_sequence

size

780

species

9031

Gallus gallus