This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET15b/ECFP-GgVcl 884-1066 (Vt)
catalog :
46180
product information
Catalog Number :
46180
Product Name :
pET15b/ECFP-GgVcl 884-1066 (Vt)
article :
doi
id6882
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepET-15b
backbone_mutation
backbone_originEMD
backbone_size5700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
CFP subcloned into 15b/V884-1066 (Addgene plasmid 46176) via a PCR strategy which placed Nde sites at the ends of the CFP cDNA and added the spacer region in EGFPC1/V1-1066 at the Cterminus of CFP.
origin :
37
pi :
alt_names
Vinculin
Vcl
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5Nde1
promoterT7
sequencing_primer_3T7 terminal
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesVINC1
geneVCL
id396422
genbank_ids
NM_205441.1
mutationaa 884-1066 (tail domain)
nameVcl 884-1066
shRNA_sequence
size555
species
9031
Gallus gallus
tags
locationN terminal on backbone
tagHis
locationN terminal on insert
tagECFP
resistance markers :
1616
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA