This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET15b/GgVcl 884-1066 (aka Vt)
catalog :
46176
citations: 1
| Reference |
|---|
Steimle P, Hoffert J, Adey N, Craig S. Polyphosphoinositides inhibit the interaction of vinculin with actin filaments. J Biol Chem. 1999;274:18414-20 pubmed
|
product information
Catalog Number :
46176
Product Name :
pET15b/GgVcl 884-1066 (aka Vt)
article :
| doi | 10.1074/jbc.274.26.18414 |
| id | 6880 |
| pubmed_id | 10373448 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pET-15b | |
| backbone_mutation | ||
| backbone_origin | EMD | |
| backbone_size | 5700 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
Using in-frame primers containing 5 -NdeI and 3 -XhoI sites, appropriate regions of the chicken vinculin cDNA sequence were amplified by PCR followed by addition of 3 -adenosine overhangs with Taq polymerase. The PCR products were ligated into the TOPO II plasmid (Invitrogen) and then subcloned into pET15b His tag expression vector (Novagen, Madison, WI) using NdeI and XhoI digestion of the multiple cloning site.
origin :
37
pi :
|
resistance markers :
| 1616 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments