This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET15b/GgVcl 1-851 A50I mutation
catalog :
46174
citations: 1
Reference
Cohen D, Kutscher B, Chen H, Murphy D, Craig S. A conformational switch in vinculin drives formation and dynamics of a talin-vinculin complex at focal adhesions. J Biol Chem. 2006;281:16006-15 pubmed
product information
Catalog Number :
46174
Product Name :
pET15b/GgVcl 1-851 A50I mutation
article :
doi10.1074/jbc.M600738200
id6879
pubmed_id16608855
bacterial resistance :
Ampicillin
cloning :
backbonepET-15b
backbone_mutation
backbone_originEMD
backbone_size5700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
See Addgene plasmid 46172 for wild-type cloning information. The A50I mutation was introduced by QuikChange mutagenesis (Stratagene).
origin :
37
pi :
alt_names
Vinculin
Vcl
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7 terminal
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesVINC1
geneVCL
id396422
genbank_ids
NM_205441.1
mutationaa 1-851 A50I mutation (talin binding domain mutant in vinculin head domain)
nameVcl 1-851 A50I
shRNA_sequence
size2559
species
9031
Gallus gallus
tags
locationN terminal on backbone
tagHis
resistance markers :
1616
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA