pET15b/GgVcl V1-258 (aka VD1) | Addgene 46173 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET15b/GgVcl V1-258 (aka VD1)

catalog :

46173

citations: 1

Reference
Cohen D, Chen H, Johnson R, Choudhury B, Craig S. Two distinct head-tail interfaces cooperate to suppress activation of vinculin by talin. J Biol Chem. 2005;280:17109-17 pubmed

product information

Catalog Number :

46173

Product Name :

pET15b/GgVcl V1-258 (aka VD1)

article :

doi	10.1074/jbc.M414704200
id	6878
pubmed_id	15728584

bacterial resistance :

Ampicillin

cloning :

backbone

pET-15b

backbone_mutation

backbone_origin

EMD

backbone_size

5700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Using in-frame primers containing 5 -NdeI and 3 -XhoI sites, appropriate regions of the chicken vinculin cDNA sequence were amplified by PCR followed by addition of 3 -adenosine overhangs with Taq polymerase. The PCR products were ligated into the TOPO II plasmid (Invitrogen) and then subcloned into pET15b His tag expression vector (Novagen, Madison, WI) using NdeI and XhoI digestion of the multiple cloning site.

origin :

pi :

alt_names

Vinculin

Vcl

cloning

clone_method	TOPO Cloning
cloning_site_3
cloning_site_5
promoter	T7
sequencing_primer_3	T7 terminal
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	VINC1
gene	VCL
id	396422

genbank_ids

NM_205441.1

mutation

aa 1-258 (VD1)

name

Vcl 1-258

shRNA_sequence

size

780

species

9031

Gallus gallus