This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PU6::unc-119_sgRNA
catalog :
46169
citations: 25
Reference
Mathies L, Kim A, Soukup E, Thomas A, Bettinger J. PBRM-1/PBAF-regulated genes in a multipotent progenitor in C. elegans. G3 (Bethesda). 2023;: pubmed publisher
Wu Y, Ding C, Weinreb A, Manning L, Swaim G, Yogev S, et al. Polarized localization of kinesin-1 and RIC-7 drives axonal mitochondria anterograde transport. bioRxiv. 2023;: pubmed publisher
Mathies L, Blackwell G, Bettinger J. New alleles of the SWI/SNF chromatin remodeling complex gene phf-10. MicroPubl Biol. 2022;2022: pubmed publisher
Goetsch P, Strome S. DREAM interrupted: severing LIN-35-MuvB association in Caenorhabditis elegans impairs DREAM function but not its chromatin localization. Genetics. 2022;221: pubmed publisher
Singh R, Smit R, Wang X, Wang C, Racher H, Hansen D. Reduction of Derlin activity suppresses Notch-dependent tumours in the C. elegans germ line. PLoS Genet. 2021;17:e1009687 pubmed publisher
Ihara S. N-terminally endogenous mKate2-tagged HIM-4 in Caenorhabditis elegans. MicroPubl Biol. 2021;2021: pubmed publisher
Zhang X, Lv S, Luo Z, Hu Y, Peng X, Lv J, et al. MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo. Nucleic Acids Res. 2021;49:4171-4185 pubmed publisher
Kuo C, Hsu Y, Wang S, Liou B, Lim S, Chen Y, et al. IGLR-2, a Leucine-Rich Repeat Domain Containing Protein, Is Required for the Host Defense in Caenorhabditis elegans. Front Immunol. 2020;11:561337 pubmed publisher
Zein Sabatto H, Cole T, Hoang H, Tiwary E, Chang C, Miller M. The type II integral ER membrane protein VAP-B homolog in C. elegans is cleaved to release the N-terminal MSP domain to signal non-cell-autonomously. Dev Biol. 2021;470:10-20 pubmed publisher
Narimatsu T, Ihara S. New allele of C. elegans gene pign-1, named as xyz11. MicroPubl Biol. 2019;2019: pubmed publisher
Culp E, Richman C, Sharanya D, Jhaveri N, van den Berg W, Gupta B. Genome editing in the nematode Caenorhabditis briggsae using the CRISPR/Cas9 system. Biol Methods Protoc. 2020;5:bpaa003 pubmed publisher
Cabral G, Laos T, Dumont J, Dammermann A. Differential Requirements for Centrioles in Mitotic Centrosome Growth and Maintenance. Dev Cell. 2019;50:355-366.e6 pubmed publisher
Loucks C, Park K, Walker D, McEwan A, Timbers T, Ardiel E, et al. EFHC1, implicated in juvenile myoclonic epilepsy, functions at the cilium and synapse to modulate dopamine signaling. elife. 2019;8: pubmed publisher
Aoki I, Tateyama M, Shimomura T, Ihara K, Kubo Y, Nakano S, et al. SLO potassium channels antagonize premature decision making in C. elegans. Commun Biol. 2018;1:123 pubmed publisher
Chen X, Shibata A, Hendi A, Kurashina M, Fortes E, Weilinger N, et al. Rap2 and TNIK control Plexin-dependent tiled synaptic innervation in C. elegans. elife. 2018;7: pubmed publisher
Uebel C, Anderson D, Mandarino L, Manage K, Aynaszyan S, Phillips C. Distinct regions of the intrinsically disordered protein MUT-16 mediate assembly of a small RNA amplification complex and promote phase separation of Mutator foci. PLoS Genet. 2018;14:e1007542 pubmed publisher
Chen B, Liu P, Hujber E, Li Y, Jorgensen E, Wang Z. AIP limits neurotransmitter release by inhibiting calcium bursts from the ryanodine receptor. Nat Commun. 2017;8:1380 pubmed publisher
Fernández Cárdenas L, Villanueva Chimal E, Salinas L, José Núñez C, Tuena de Gomez Puyou M, Navarro R. Caenorhabditis elegans ATPase inhibitor factor 1 (IF1) MAI-2 preserves the mitochondrial membrane potential (??m) and is important to induce germ cell apoptosis. PLoS ONE. 2017;12:e0181984 pubmed publisher
Cottee P, Cole T, Schultz J, Hoang H, Vibbert J, Han S, et al. The C. elegans VAPB homolog VPR-1 is a permissive signal for gonad development. Development. 2017;144:2187-2199 pubmed publisher
Schultz J, Lee S, Cole T, Hoang H, Vibbert J, Cottee P, et al. The secreted MSP domain of C. elegans VAPB homolog VPR-1 patterns the adult striated muscle mitochondrial reticulum via SMN-1. Development. 2017;144:2175-2186 pubmed publisher
Raman P, Zaghab S, Traver E, Jose A. The double-stranded RNA binding protein RDE-4 can act cell autonomously during feeding RNAi in C. elegans. Nucleic Acids Res. 2017;45:8463-8473 pubmed publisher
Choi Y, Edwards L, DiBello A, Jose A. Removing bias against short sequences enables northern blotting to better complement RNA-seq for the study of small RNAs. Nucleic Acids Res. 2017;45:e87 pubmed publisher
Schwartz M, Jorgensen E. SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans. Genetics. 2016;202:1277-88 pubmed publisher
Katic I, Xu L, Ciosk R. CRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair. G3 (Bethesda). 2015;5:1649-56 pubmed publisher
Friedland A, Tzur Y, Esvelt K, COLAIACOVO M, Church G, Calarco J. Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Nat Methods. 2013;10:741-3 pubmed publisher
product information
Catalog Number :
46169
Product Name :
PU6::unc-119_sgRNA
article :
doi10.1038/nmeth.2532
id6972
pubmed_id23817069
bacterial resistance :
Ampicillin
cloning :
backbonepUC57
backbone_mutation
backbone_origin
backbone_size2641
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
growth notes :
For more information on Calarco Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/calarco/ gRNA target sequence GAATTTTCTGAAATTAAAGA
origin :
37
pi :
alt_names
unc-119
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5EcoRI
promoterC. elegans U6 snRNA pol III promoter
sequencing_primer_3M13 (-48) reverse
sequencing_primer_5M13 (-40) forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_M142.1
geneunc-119
id176519
genbank_ids
mutation
nameunc-119 targeting sgRNA
shRNA_sequence
size840
species
100
Synthetic
tags
resistance markers :
1609
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA