This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pT3-EFa1-HA-Jag1
catalog :
46051
citations: 1
Reference
Tschaharganeh D, Chen X, Latzko P, Malz M, Gaida M, Felix K, et al. Yes-associated protein up-regulates Jagged-1 and activates the Notch pathway in human hepatocellular carcinoma. Gastroenterology. 2013;144:1530-1542.e12 pubmed publisher
product information
Catalog Number :
46051
Product Name :
pT3-EFa1-HA-Jag1
article :
doi10.1053/j.gastro.2013.02.009
id6857
pubmed_id23419361
bacterial resistance :
Ampicillin
cloning :
backbonepT3-EF1a
backbone_mutation
backbone_origin
backbone_size7000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This plasmid is in the pT3-EF1a vector which contains loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid cannot not used with Cre for in vivo studies.
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterEF1a
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneJag1
id29146
genbank_ids
mutation
nameJag1
shRNA_sequence
size3783
species
10116
Rattus norvegicus
tags
locationC terminal on insert
tagHA tag
plasmid copy :
Jag1 gene from Dr Gerry Weinmaster s lab, UCLA.
resistance markers :
1021
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA