pT3-EF1a-NICD1 | Addgene 46047 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pT3-EF1a-NICD1

catalog :

46047

citations: 3

Reference
Jarman E, Horcas Lopez M, Waddell S, MacMaster S, Gournopanos K, Soong D, et al. DKK1 drives immune suppressive phenotypes in intrahepatic cholangiocarcinoma and can be targeted with anti-DKK1 therapeutic DKN-01. Liver Int. 2023;43:208-220 pubmed publisher
Bao X, Li Q, Chen J, Chen D, Ye C, Dai X, et al. Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing-Assisted Multiomics Analysis. Cancer Immunol Res. 2022;10:811-828 pubmed publisher
Fan B, Malato Y, Calvisi D, Naqvi S, Razumilava N, Ribback S, et al. Cholangiocarcinomas can originate from hepatocytes in mice. J Clin Invest. 2012;122:2911-5 pubmed publisher

product information

Catalog Number :

46047

Product Name :

pT3-EF1a-NICD1

article :

doi	10.1172/JCI63212
id	6856
pubmed_id	22797301

bacterial resistance :

Ampicillin

cloning :

backbone

pT3-EF1a

backbone_mutation

backbone_origin

backbone_size

7000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

This plasmid is in the pT3-EF1a vector which contains loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid cannot not used with Cre for in vivo studies. For in vivo use in mice with Cre, please use plasmid 86500.

origin :

pi :

alt_names

Notch 1

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	EF1a
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	9930111A19Rik, Mis6, N1, Tan1, lin-12
gene	Notch1
id	18128

genbank_ids

mutation

Cytoplasmic domain initiating at amino acid 1753

name

Notch1 (C-term)

shRNA_sequence

size

2385

species

10090

Mus musculus