This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-parkin F146A
catalog :
45973
citations: 1
Reference
Trempe J, Sauvé V, Grenier K, Seirafi M, Tang M, Ménade M, et al. Structure of parkin reveals mechanisms for ubiquitin ligase activation. Science. 2013;340:1451-5 pubmed publisher
product information
Catalog Number :
45973
Product Name :
pGEX-parkin F146A
article :
doi10.1126/science.1237908
id6823
pubmed_id23661642
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-6P-1
backbone_mutation
backbone_originAmersham
backbone_size4966
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
origin :
37
pi :
alt_names
PARK2
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promotertac
sequencing_primer_3PGEXR
sequencing_primer_5PGEXF
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPark, Park2
genePrkn
id56816
genbank_ids
mutationCodon usage optimized for E.coli expression. Changed Phe146 to alanine.
nameParkin F146A
shRNA_sequence
size1398
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagGST
plasmid copy :
DNAexpress (http://dnaxps.com) performed mutagenesis from codon optimized wild-type parkin in pGEX-6P-1.
resistance markers :
1597
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA