pHsp70-Cas9 | Addgene 45945 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pHsp70-Cas9

catalog :

45945

citations: 14

Reference
Ham S, Yoo H, Woo D, Lee D, Park K, Chung J. PINK1 and Parkin regulate IP3R-mediated ER calcium release. Nat Commun. 2023;14:5202 pubmed publisher
Langm xfc ller A, Champer J, Lapinska S, Xie L, Metzloff M, Champer S, et al. Fitness effects of CRISPR endonucleases in Drosophila melanogaster populations. elife. 2022;11: pubmed publisher
Auer T, xc1 lvarez Oca xf1 a R, Cruchet S, Benton R, Arguello J. Copy number changes in co-expressed odorant receptor genes enable selection for sensory differences in drosophilid species. Nat Ecol Evol. 2022;6:1343-1353 pubmed publisher
Metzloff M, Yang E, Dhole S, Clark A, Messer P, Champer J. Experimental demonstration of tethered gene drive systems for confined population modification or suppression. BMC Biol. 2022;20:119 pubmed publisher
Yang E, Metzloff M, Langmüller A, Xu X, Clark A, Messer P, et al. A homing suppression gene drive with multiplexed gRNAs maintains high drive conversion efficiency and avoids functional resistance alleles. G3 (Bethesda). 2022;12: pubmed publisher
Ham S, Lee D, Xu W, Cho E, Choi S, Min S, et al. Loss of UCHL1 rescues the defects related to Parkinson's disease by suppressing glycolysis. Sci Adv. 2021;7: pubmed publisher
Carvajal Garcia J, Crown K, Ramsden D, Sekelsky J. DNA polymerase theta suppresses mitotic crossing over. PLoS Genet. 2021;17:e1009267 pubmed publisher
Lule Chávez A, Carballar Lejarazú R, Cabrera Ponce J, Lanz Mendoza H, Ibarra J. Genetic transformation of mosquitoes by microparticle bombardment. Insect Mol Biol. 2021;30:30-41 pubmed publisher
Champer J, Lee E, Yang E, Liu C, Clark A, Messer P. A toxin-antidote CRISPR gene drive system for regional population modification. Nat Commun. 2020;11:1082 pubmed publisher
Champer J, Liu J, Oh S, Reeves R, Luthra A, Oakes N, et al. Reducing resistance allele formation in CRISPR gene drive. Proc Natl Acad Sci U S A. 2018;115:5522-5527 pubmed publisher
Xu X, GANTZ V, Siomava N, Bier E. CRISPR/Cas9 and active genetics-based trans-species replacement of the endogenous Drosophila kni-L2 CRM reveals unexpected complexity. elife. 2017;6: pubmed publisher
Champer J, Reeves R, Oh S, Liu C, Liu J, Clark A, et al. Novel CRISPR/Cas9 gene drive constructs reveal insights into mechanisms of resistance allele formation and drive efficiency in genetically diverse populations. PLoS Genet. 2017;13:e1006796 pubmed publisher
Dong S, Lin J, Held N, Clem R, Passarelli A, Franz A. Heritable CRISPR/Cas9-mediated genome editing in the yellow fever mosquito, Aedes aegypti. PLoS ONE. 2015;10:e0122353 pubmed publisher
Gratz S, Cummings A, Nguyen J, Hamm D, Donohue L, Harrison M, et al. Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Genetics. 2013;194:1029-35 pubmed publisher

product information

Catalog Number :

45945

Product Name :

pHsp70-Cas9

article :

doi	10.1534/genetics.113.152710
id	6834
pubmed_id	23709638

bacterial resistance :

Kanamycin

cloning :

backbone

pHSS6

backbone_mutation

Cas9 was cloned into pHSS6hsILMi20 (Pavlopoulos et al., 2004) between the hsp70 promoter and 3'UTR using ClaI (5') and XbaI (3').

backbone_origin

backbone_size

2300

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

CRISPR

growth notes :

For more information on FlyCRISPR Plasmids and a list of related plasmids please refer to: http://www.addgene.org/crispr/OConnor-Giles/ Plasmid 51019: pDsRed-attP (www.addgene.org/51019) can be for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site.

growth strain :

The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

origin :

pi :

alt_names

SpCas9

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	ClaI
promoter
sequencing_primer_3	cccatatgttataacccattgatgaac
sequencing_primer_5	ctgcagtaaagtgcaagttaaagtg
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

Silent change from C to A at bp 834 of insert

name

Codon optimized Cas9

shRNA_sequence

size

4272

species