This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCRII-Topo Tmtc4
catalog :
45869
citations: 1
Reference
Molyneaux B, Arlotta P, Fame R, MacDonald J, MacQuarrie K, Macklis J. Novel subtype-specific genes identify distinct subpopulations of callosal projection neurons. J Neurosci. 2009;29:12343-54 pubmed publisher
product information
Catalog Number :
45869
Product Name :
pCRII-Topo Tmtc4
article :
doi10.1523/JNEUROSCI.6108-08.2009
id6757
pubmed_id19793993
bacterial resistance :
Ampicillin
cloning :
backbonepCRII-Topo
backbone_mutation
backbone_originInvitrogen
backbone_size4000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
in situ
growth notes :
The Tmtc4 fragment was amplified by RT-PCR using the following primer pair: GAAGCAGAGCAGAGCTACCG TCTGAACAGAGGCTTCATGC For in vitro transcription, use the Spe1 restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation.
origin :
37
pi :
alt_names
TMTC4
j22riken
transmembrane and tetratricopeptide repeat containing 4
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3T7, M13 Forward
sequencing_primer_5M13 Rev, Sp6
site_3_destroyed
site_5_destroyed
entrez_gene
aliases4930403J22Rik, 5730419O14Rik
geneTmtc4
id70551
genbank_ids
NM_028651
mutationnt 1220-1798 on BC031368
nameTMTC4 in situ probe
shRNA_sequence
size579
species
10090
Mus musculus
tags
resistance markers :
1573
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA