pCRII-Topo Frmd4b | Addgene 45867 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCRII-Topo Frmd4b

catalog :

45867

citations: 1

Reference
Molyneaux B, Arlotta P, Fame R, MacDonald J, MacQuarrie K, Macklis J. Novel subtype-specific genes identify distinct subpopulations of callosal projection neurons. J Neurosci. 2009;29:12343-54 pubmed publisher

product information

Catalog Number :

45867

Product Name :

pCRII-Topo Frmd4b

article :

doi	10.1523/JNEUROSCI.6108-08.2009
id	6757
pubmed_id	19793993

bacterial resistance :

Ampicillin

cloning :

backbone

pCRII-Topo

backbone_mutation

backbone_origin

Invitrogen

backbone_size

4000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

in situ

growth notes :

The Frmd4b fragment was amplified by RT-PCR using the following primer pair: AGCTCCTGAATCGTGGCTTA TCCTGCAGCTCGGAGTAAAT For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation. Please note that Addgene's sequencing results match bp# 3933-4535 of NM_145148.2, not bp# 3856-4458 as indicated on plasmid map as indicated on plasmid map as indicated on plasmid map. The plasmid functions as described in the associated publication.

origin :

pi :

alt_names

Frmd4b

Grsp1

GSP

cloning

clone_method	TOPO Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	T7, M13 Forward
sequencing_primer_5	M13 Rev, Sp6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	6030440G05Rik, C87375, GOBLIN, GRSP1, R74720
gene	Frmd4b
id	232288

genbank_ids

NM_145148

mutation

contains bp# 3933-4535 of NM_145148.2

name

Frmd4b in situ probe

shRNA_sequence

size

603

species

10090

Mus musculus