This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLPCX-PECAMTL
catalog :
45851
citations: 1
Reference
Conway D, Breckenridge M, Hinde E, Gratton E, Chen C, Schwartz M. Fluid shear stress on endothelial cells modulates mechanical tension across VE-cadherin and PECAM-1. Curr Biol. 2013;23:1024-30 pubmed publisher
product information
Catalog Number :
45851
Product Name :
pLPCX-PECAMTL
article :
doi10.1016/j.cub.2013.04.049
id6811
pubmed_id23684974
bacterial resistance :
Ampicillin
cloning :
backbonepLPCX
backbone_mutation
backbone_originClonetech
backbone_size6265
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Note: Compared to the current NCBI reference sequence for PECAM1, this plasmid has a V125L substitution. This is a known SNP. Also, two silent mutations were introduced to destroy two internal EcoRI sites.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3ACCTACAGGTGGGGTCTTTCATTCCC
sequencing_primer_5AGCTCGTTTAGTGAACCGTCAGATC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD31, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, endoCAM
genePECAM1
id5175
genbank_ids
mutation
namePECAM1
shRNA_sequence
size3800
species
9606
Homo sapiens
tags
resistance markers :
451
tags :
Low Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA