This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
a1Ic-HE3-pcDNA3
catalog :
45810
citations: 5
Reference
Hackney C, Fl xf3 rez Salcedo P, Mueller E, Koch T, Kjelgaard L, Watkins M, et al. A previously unrecognized superfamily of macro-conotoxins includes an inhibitor of the sensory neuron calcium channel Cav2.3. PLoS Biol. 2023;21:e3002217 pubmed publisher
McArthur J, Wen J, Hung A, Finol Urdaneta R, Adams D. µ-Theraphotoxin Pn3a inhibition of CaV3.3 channels reveals a novel isoform-selective drug binding site. elife. 2022;11: pubmed publisher
Zhao Y, Sivaji S, Chiang M, Ali H, Zukowski M, Ali S, et al. Amyloid Beta Peptides Block New Synapse Assembly by Nogo Receptor-Mediated Inhibition of T-Type Calcium Channels. Neuron. 2017;96:355-372.e6 pubmed publisher
McArthur J, Motin L, Gleeson E, Spiller S, Lewis R, Duggan P, et al. Inhibition of human N- and T-type calcium channels by an ortho-phenoxyanilide derivative, MONIRO-1. Br J Pharmacol. 2018;175:2284-2295 pubmed publisher
Gomora J, Murbartián J, Arias J, Lee J, Perez Reyes E. Cloning and expression of the human T-type channel Ca(v)3.3: insights into prepulse facilitation. Biophys J. 2002;83:229-41 pubmed
product information
Catalog Number :
45810
Product Name :
a1Ic-HE3-pcDNA3
article :
doi10.1016/S0006-3495(02)75164-3
id6797
pubmed_id12080115
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3-HE3
backbone_mutationpcDNA3 has been modified to contain 54 bp of the 5 untranslated region of Xenopus beta globin. Previous studies have shown that this sequence can significantly enhance expression in Xenopus oocytes (Liman et al., 1992), and does not interfere with expression in mammalian cells (Lee et al., 1999).
backbone_originPerez-Reyes
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
making cRNA for oocyte injections
growth notes :
coding region ends at 6568
origin :
37
pi :
alt_names
Cav3.3c
calcium channel, voltage-dependent, T type, alpha 1I subunit
LT9
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3BGHrev
sequencing_primer_5CMV-F; T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCav3.3, ca(v)3.3
geneCACNA1I
id8911
genbank_ids
AF393329
mutation
nameCACNA1I
shRNA_sequence
size7700
species
9606
Homo sapiens
tags
resistance markers :
1584
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA