This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
1Ic-HE2-pcDNA3
catalog :
45808
citations: 1
Reference
Murbartián J, Arias J, Lee J, Gomora J, Perez Reyes E. Alternative splicing of the rat Ca(v)3.3 T-type calcium channel gene produces variants with distinct functional properties(1). FEBS Lett. 2002;528:272-8 pubmed
product information
Catalog Number :
45808
Product Name :
1Ic-HE2-pcDNA3
article :
doi10.1016/S0014-5793(02)03341-0
id6795
pubmed_id12297319
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3-HE2
backbone_mutationpcDNA3 has been modified to contain 54 bp of the 5 untranslated region of Xenopus beta globin. Previous studies have shown that this sequence can significantly enhance expression in Xenopus oocytes (Liman et al., 1992), and does not interfere with expression in mammalian cells (Lee et al., 1999).
backbone_originPerez-Reyes
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
cav3.3c
calcium channel, voltage-dependent, T type, alpha 1I subunit
Cacna1i
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3BGHrev
sequencing_primer_5CMV-F; T7
site_3_destroyed
site_5_destroyed1
entrez_gene
aliases
geneCacna1i
id56827
genbank_ids
AY128645
mutation
name 1Ic
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
resistance markers :
1584
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA