This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
IL2R/hE-cadherin-cytotail
catalog :
45773
citations: 2
Reference
Reppschläger K, Gosselin J, Dangelmaier C, Thomas D, Carpino N, McKenzie S, et al. TULA-2 Protein Phosphatase Suppresses Activation of Syk through the GPVI Platelet Receptor for Collagen by Dephosphorylating Tyr(P)346, a Regulatory Site of Syk. J Biol Chem. 2016;291:22427-22441 pubmed
Gottardi C, Wong E, Gumbiner B. E-cadherin suppresses cellular transformation by inhibiting beta-catenin signaling in an adhesion-independent manner. J Cell Biol. 2001;153:1049-60 pubmed
product information
Catalog Number :
45773
Product Name :
IL2R/hE-cadherin-cytotail
article :
doi10.1083/jcb.153.5.1049
id6786
pubmed_id11381089
bacterial resistance :
Ampicillin
cloning :
backbonepCMV
backbone_mutation
backbone_originInvitrogen
backbone_size5446
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This IL2R/E-cadherin cytoplasmic chimera fuses the extracellular and transmembrane domains of the interleukin-2 receptor subunit to the cytoplasmic domain of E-cadherin. This construct can bind -catenin but cannot engage in homophilic adhesive activity.
origin :
37
pi :
alt_names
Interleukin-2 receptor subunit extracellular and transmembrane domains
E-cadherin cytoplasmic domain
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5NheI
promoterCMV
sequencing_primer_3Unknown
sequencing_primer_5CMV-F; T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD25, IDDM10, IL2R, IMD41, TCGFR, p55
geneIL2RA
id3559
aliasesArc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO
geneCDH1
id999
genbank_ids
mutationInterleukin-2 receptor subunit extracellular and transmembrane domains fused to E-cadherin cytoplasmic domain
nameIL2R/hE-cadherin-cytotail
shRNA_sequence
size
species
9606
Homo sapiens
tags
plasmid copy :
Human E-cadherin partial cDNA provided by David Rimm (Yale University, New Haven, CT). IL2R-1 subunit cDNA from pCMV-IL2R-1 from Susan LaFlamme (Albany Medical College, Albany, NY), originally from Anthony Giordano (Giordano et al ., 1991)
resistance markers :
181
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA