This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
hE-cadherin/ -catenin-pcDNA3
catalog :
45772
citations: 1
| Reference |
|---|
Gottardi C, Wong E, Gumbiner B. E-cadherin suppresses cellular transformation by inhibiting beta-catenin signaling in an adhesion-independent manner. J Cell Biol. 2001;153:1049-60 pubmed
|
product information
Catalog Number :
45772
Product Name :
hE-cadherin/ -catenin-pcDNA3
article :
| doi | 10.1083/jcb.153.5.1049 |
| id | 6786 |
| pubmed_id | 11381089 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pcDNA3 | |
| backbone_mutation | ||
| backbone_origin | Invitrogen | |
| backbone_size | 5446 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
Partial cDNA for human E-cadherin was provided by D. Rimm (Yale University, New Haven, CT) and subcloned into the pcDNA3 mammalian expression vector (Invitrogen). Sequence analysis revealed that the 3 end of the gene was missing after nucleotide 2644 (according to EMBL/GenBank/DDBJ under accession number L08599). This results in a truncation of the last 35 amino acids of the E-cadherin cytoplasmic domain and, as a result, does not contain the -catenin binding region, as defined by Stappert and Kemler 1994. The COOH terminus of this truncation mutant (E-cadherin -catenin) ends at amino acid 847 (NH3-ASLSST); the frameshift adds a single threonine residue before a stop codon is introduced.
origin :
37
pi :
|
plasmid copy :
Human E-cadherin partial cDNA provided by David Rimm (Yale University, New Haven, CT)
resistance markers :
| 181 |
tags :
High Copy
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments