This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
hE-cadherin/ -catenin fusion-pcDNA3
catalog :
45771
citations: 1
| Reference |
|---|
Gottardi C, Wong E, Gumbiner B. E-cadherin suppresses cellular transformation by inhibiting beta-catenin signaling in an adhesion-independent manner. J Cell Biol. 2001;153:1049-60 pubmed
|
product information
Catalog Number :
45771
Product Name :
hE-cadherin/ -catenin fusion-pcDNA3
article :
| doi | 10.1083/jcb.153.5.1049 |
| id | 6786 |
| pubmed_id | 11381089 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pcDNA3 | |
| backbone_mutation | ||
| backbone_origin | Invitrogen | |
| backbone_size | 5446 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
For this E-cadherin -catenin fusion construct that lacks the -catenin binding domain in both E-cadherin and -catenin, cDNA encoding the membrane proximal region of the cadherin cytoplasmic domain was amplified between nucleotides 1760 and 2530 using 5 (5 -TGAGCACGTGAAGAACAGCACGTACAC-3 ) and 3 (5 -CCCTGGCCATTTCCAATTTCATCGGGA-3 ) oligonucleotide primers, and the resultant 770-bp fragment was cloned into a Xenopus -catenin cDNA such that amino acid 809 in E-cadherin was fused in frame with amino acid 201 in -catenin (E-cad-DEIGN/GHRDQ- -catenin). This fusion region was further subcloned into the E-cad/pcDNA3 plasmid (Addgene plasmid 45769) to make the E-cadherin -catenin cDNA.
origin :
37
pi :
|
plasmid copy :
Human E-cadherin partial cDNA provided by David Rimm (Yale University, New Haven, CT)
resistance markers :
| 181 |
tags :
High Copy
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
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