hE-cadherin-pcDNA3 | Addgene 45769 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

hE-cadherin-pcDNA3

catalog :

45769

citations: 13

Reference
Li J, Sun H, Wang M, Chen P. E-cadherin Interacts With Posttranslationally-Modified AGO2 to Enhance miRISC Activity. Front Cell Dev Biol. 2021;9:671244 pubmed publisher
Förster A, Brand F, Banan R, Hüneburg R, Weber C, Ewert W, et al. Rare germline variants in the E-cadherin gene CDH1 are associated with the risk of brain tumors of neuroepithelial and epithelial origin. Acta Neuropathol. 2021;142:191-210 pubmed publisher
Urushima H, Yuasa H, Matsubara T, Kuroda N, Hara Y, Inoue K, et al. Activation of Hepatic Stellate Cells Requires Dissociation of E-Cadherin-Containing Adherens Junctions with Hepatocytes. Am J Pathol. 2020;: pubmed publisher
Tasdemir N, Ding K, Savariau L, Levine K, Du T, Elangovan A, et al. Proteomic and transcriptomic profiling identifies mediators of anchorage-independent growth and roles of inhibitor of differentiation proteins in invasive lobular carcinoma. Sci Rep. 2020;10:11487 pubmed publisher
Kitchen P, Lee K, Clark D, Lau N, Lertsuwan J, Sawasdichai A, et al. A runaway PRH/HHEX-Notch3 positive feedback loop drives cholangiocarcinoma and determines response to CDK4/6 inhibition. Cancer Res. 2019;: pubmed publisher
Boone P, Rochelle L, Ginzel J, Lubkov V, Roberts W, Nicholls P, et al. A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion. Nat Commun. 2019;10:5490 pubmed publisher
Gaber A, Kim S, Kaake R, Bencina M, Krogan N, Sali A, et al. EpCAM homo-oligomerization is not the basis for its role in cell-cell adhesion. Sci Rep. 2018;8:13269 pubmed publisher
Daugaard I, Sanders K, Idica A, Vittayarukskul K, Hamdorf M, Krog J, et al. miR-151a induces partial EMT by regulating E-cadherin in NSCLC cells. Oncogenesis. 2017;6:e366 pubmed publisher
Wang S, Cheng Y, Zheng Y, He Z, Chen W, Zhou W, et al. PRKAR1A is a functional tumor suppressor inhibiting ERK/Snail/E-cadherin pathway in lung adenocarcinoma. Sci Rep. 2016;6:39630 pubmed publisher
Song H, Zhang Y, Liu N, Zhao S, Kong Y, Yuan L. miR-92a-3p Exerts Various Effects in Glioma and Glioma Stem-Like Cells Specifically Targeting CDH1/?-Catenin and Notch-1/Akt Signaling Pathways. Int J Mol Sci. 2016;17: pubmed
Xiong S, Klausen C, Cheng J, Leung P. Activin B promotes endometrial cancer cell migration by down-regulating E-cadherin via SMAD-independent MEK-ERK1/2-SNAIL signaling. Oncotarget. 2016;7:40060-40072 pubmed publisher
Cui T, Srivastava A, Han C, Yang L, Zhao R, Zou N, et al. XPC inhibits NSCLC cell proliferation and migration by enhancing E-Cadherin expression. Oncotarget. 2015;6:10060-72 pubmed
Gottardi C, Wong E, Gumbiner B. E-cadherin suppresses cellular transformation by inhibiting beta-catenin signaling in an adhesion-independent manner. J Cell Biol. 2001;153:1049-60 pubmed

product information

Catalog Number :

45769

Product Name :

hE-cadherin-pcDNA3

article :

doi	10.1083/jcb.153.5.1049
id	6786
pubmed_id	11381089

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5446

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Partial cDNA for human E-cadherin was provided by D. Rimm (Yale University, New Haven, CT) and subcloned into the pcDNA3 mammalian expression vector (Invitrogen), to generate Addgene plasmid 45772. Sequence analysis revealed that the 3 end of the gene was missing after nucleotide 2644 (according to EMBL/GenBank/DDBJ under accession number L08599). This resulted in a truncation of the last 35 amino acids of the E-cadherin cytoplasmic domain and, as a result, did not contain the -catenin binding region, as defined by Stappert and Kemler 1994. The COOH terminus of this truncation mutant (E-cadherin -catenin) ends at amino acid 844 (NH3-ASLSSD); the frameshift added a single histidine residue before a stop codon is introduced. The full-length human E-cadherin was reengineered using RNA from human A431 cells and the RT-PCR method (Primer A, 5 -TGACACCCGGGACAACGTTTATTA-3 , and Primer C, 5 -CTAGTCTAGACCCCTAGTGGTCCTCG-3 ) to generate a 425-bp fragment encoding the missing COOH-terminal residues. This fragment was sub-cloned into the truncated hEcad- 35/pcDNA3 vector (Addgene plasmid 45772) to generate full-length hEcad/pcDNA3 (Addgene plasmid 45769).

origin :

pi :

alt_names

E-cadherin

CDH-1

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	HindIII
promoter	CMV
sequencing_primer_3	BGH-rev; Sp6
sequencing_primer_5	CMV-F; T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO
gene	CDH1
id	999

genbank_ids

NM_004360.4

NP_004351.1

mutation

name

hE-cadherin

shRNA_sequence

size

3100

species

9606

Homo sapiens