pcDNA3.1 AT1R DRY-AAY | Addgene 45759 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3.1 AT1R DRY-AAY

catalog :

45759

citations: 2

Reference
Zaccor N, Sumner C, Snyder S. The nonselective cation channel TRPV4 inhibits angiotensin II receptors. J Biol Chem. 2020;295:9986-9997 pubmed publisher
Gaborik Z, Jagadeesh G, Zhang M, Spät A, Catt K, Hunyady L. The role of a conserved region of the second intracellular loop in AT1 angiotensin receptor activation and signaling. Endocrinology. 2003;144:2220-8 pubmed

product information

Catalog Number :

45759

Product Name :

pcDNA3.1 AT1R DRY-AAY

article :

doi	10.1210/en.2002-0135
id	6783
pubmed_id	12746278

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1

backbone_mutation

backbone_origin

Invitrogen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The cDNA of the rat vascular smooth muscle AT1A receptor was donated by Dr. K. E. Bernstein (Emory University, Atlanta, GA). Mutations in the rat AT1A receptor were performed with the Mutagene kit(Bio-Rad Laboratories, Inc., Hercules, CA) N4D mutation is present to prevent glycosylation at this consensus site and steric hindrance of antibody binding.

origin :

pi :

alt_names

AT1AR

angiotensin II receptor, type 1a

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	BGHrev
sequencing_primer_5	CMV-F; T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AT1, AT1A, AT1R, Agtr1
gene	Agtr1a
id	24180

genbank_ids

mutation

changed amino acids 125-126 from DR to AA

name

AT1R DRY-AAY

shRNA_sequence

size

species

10116

Rattus norvegicus