pcDNA3 HA eIF4GI (1-1599) | Addgene 45640 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3 HA eIF4GI (1-1599)

catalog :

45640

citations: 6

Reference
Dabrowska A, Milewska A, Ner Kluza J, Suder P, Pyrc K. Mass Spectrometry versus Conventional Techniques of Protein Detection: Zika Virus NS3 Protease Activity towards Cellular Proteins. Molecules. 2021;26: pubmed publisher
Dong Z, Chen W, Chen C, Wang H, Cui W, Tan Z, et al. CUL3 Deficiency Causes Social Deficits and Anxiety-like Behaviors by Impairing Excitation-Inhibition Balance through the Promotion of Cap-Dependent Translation. Neuron. 2019;: pubmed publisher
Rigogliuso G, Biniossek M, Goodier J, Mayer B, Pereira G, Schilling O, et al. A human endogenous retrovirus encoded protease potentially cleaves numerous cellular proteins. Mob DNA. 2019;10:36 pubmed publisher
Jo S, Lockridge A, Alejandro E. eIF4G1 and carboxypeptidase E axis dysregulation in O-GlcNAc transferase-deficient pancreatic β-cells contributes to hyperproinsulinemia in mice. J Biol Chem. 2019;294:13040-13050 pubmed publisher
Grzmil M, Seebacher J, Hess D, Behe M, Schibli R, Moncayo G, et al. Inhibition of MNK pathways enhances cancer cell response to chemotherapy with temozolomide and targeted radionuclide therapy. Cell Signal. 2016;28:1412-21 pubmed publisher
Yanagiya A, Svitkin Y, Shibata S, Mikami S, Imataka H, Sonenberg N. Requirement of RNA binding of mammalian eukaryotic translation initiation factor 4GI (eIF4GI) for efficient interaction of eIF4E with the mRNA cap. Mol Cell Biol. 2009;29:1661-9 pubmed publisher

product information

Catalog Number :

45640

Product Name :

pcDNA3 HA eIF4GI (1-1599)

article :

doi	10.1128/MCB.01187-08
id	6762
pubmed_id	19114555

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

For diagnostic digest, an additional PvuI digestion is necessary to distinguish the insert from the vector because of their similar sizes. The EIF4G1 translation contains M432V compared to NP_937884 (NM_198241.2); however the nucleotide change resulting in the amino acid change is more conserved between species, and it is located between functional domains of eIF4G1 (PABP-binding site and eIF4E-binding site), and does not affect its function as a translational factor

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	HindIII
promoter	CMV
sequencing_primer_3	BGH-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	EIF-4G1, EIF4F, EIF4G, EIF4GI, P220, PARK18
gene	EIF4G1
id	1981

genbank_ids

NM_198241

mutation

name

eIF4GI transcript variant 2

shRNA_sequence

size

4900

species

9606

Homo sapiens