pCDNA3.1 AT1AR-324delta | Addgene 45635 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCDNA3.1 AT1AR-324delta

catalog :

45635

citations: 1

Reference
Wei H, Ahn S, Barnes W, Lefkowitz R. Stable interaction between beta-arrestin 2 and angiotensin type 1A receptor is required for beta-arrestin 2-mediated activation of extracellular signal-regulated kinases 1 and 2. J Biol Chem. 2004;279:48255-61 pubmed

product information

Catalog Number :

45635

Product Name :

pCDNA3.1 AT1AR-324delta

article :

doi	10.1074/jbc.M406205200
id	6759
pubmed_id	15355986

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1/Zeo

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Mutant AT1A receptors were generated by mutagenesis PCR using pcDNA3.1-HA-AT1A receptor (provided by M. G. Caron, Duke University) as a template and a QuikChange multisite-directed mutagenesis kit (Stratagene) according to the manufacturer s instructions. The primer for the 324delta receptor was: 5' p-CCCCCAAAGGCCTAGCTCCCACTCAAGCC 3'. N4D mutation introduced to prevent glycosylation at this consensus site and steric hindrance of antibody binding.

origin :

pi :

alt_names

AT1AR

Agtr1

angiotensin II receptor, type 1

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	HindIII
promoter	CMV
sequencing_primer_3	BGH-rev
sequencing_primer_5	CMV-F; T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AT1, AT1A, AT1R, Agtr1
gene	Agtr1a
id	24180

genbank_ids

mutation

added stop codon at 324 aa

name

AT1AR-324delta

shRNA_sequence

size

species

10116

Rattus norvegicus