pCRII-Topo Hspb3 in situ probe | Addgene 45629 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

pCRII-Topo Hspb3 in situ probe

catalog :

45629

citations: 1

Reference
Molyneaux B, Arlotta P, Fame R, MacDonald J, MacQuarrie K, Macklis J. Novel subtype-specific genes identify distinct subpopulations of callosal projection neurons. J Neurosci. 2009;29:12343-54 pubmed publisher

product information

Catalog Number :

45629

Product Name :

pCRII-Topo Hspb3 in situ probe

article :

doi	10.1523/JNEUROSCI.6108-08.2009
id	6757
pubmed_id	19793993

bacterial resistance :

Ampicillin

cloning :

backbone

pCRII-Topo

backbone_mutation

backbone_origin

Invitrogen

backbone_size

4000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

in situ

growth notes :

The Hspb3 fragment was amplified by RT-PCR using the following primer pair: TGATTCAGCCCCAATTAAGC CTGGGGTATGAAGAGCAACC For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation.

origin :

pi :

alt_names

heat shock protein 3

Hspb3

spb3

cloning

clone_method	TOPO Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	T7, M13 Forward
sequencing_primer_5	M13 Rev, Sp6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	2310035K17Rik, AI844863, Hsbp3, spb3
gene	Hspb3
id	56534

genbank_ids

BC065388.1

mutation

fragment contains nt 30-661 of Hspb3 mRNA sequence (BC065388.1)

name

Hspb3 in situ probe

shRNA_sequence

size

632

species

10090

Mus musculus