pCRII-Topo Tcrb-V13 in situ probe | Addgene 45622 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCRII-Topo Tcrb-V13 in situ probe

catalog :

45622

citations: 1

Reference
Molyneaux B, Arlotta P, Hirata T, Hibi M, Macklis J. Fezl is required for the birth and specification of corticospinal motor neurons. Neuron. 2005;47:817-31 pubmed

product information

Catalog Number :

45622

Product Name :

pCRII-Topo Tcrb-V13 in situ probe

article :

doi	10.1016/j.neuron.2005.08.030
id	6756
pubmed_id	16157277

bacterial resistance :

Ampicillin

cloning :

backbone

pCRII-Topo

backbone_mutation

backbone_origin

Invitrogen

backbone_size

4000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

in situ

growth notes :

The Tcrb-V13 fragment was amplified by RT-PCR using the following primer pair: CTGAGCTGGTGGGTGAATG AAGGTGTCAACGAGGAAGGA For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation. Please note that Addgene's sequencing results match bp# 533-1058 of X67128, not bp# 532-1059 as indicated on plasmid map. The plasmid functions as described in the associated publication.

origin :

pi :

alt_names

T cell receptor beta chain

TcrB

cloning

clone_method	TOPO Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	T7, M13 Forward
sequencing_primer_5	M13 Rev, Sp6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	TCRbeta, Tib
gene	Tcrb
id	21577

genbank_ids

mutation

fragment contains bp# 533-1058 of X67128

name

Tcrb-V13 in situ probe

shRNA_sequence

size

527

species

10090

Mus musculus