pCRII-Topo Diap3 in situ probe | Addgene 45602 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCRII-Topo Diap3 in situ probe

catalog :

45602

citations: 2

Reference
Damiani D, Goffinet A, ALBERTS A, Tissir F. Lack of Diaph3 relaxes the spindle checkpoint causing the loss of neural progenitors. Nat Commun. 2016;7:13509 pubmed publisher
Arlotta P, Molyneaux B, Chen J, Inoue J, Kominami R, Macklis J. Neuronal subtype-specific genes that control corticospinal motor neuron development in vivo. Neuron. 2005;45:207-21 pubmed

product information

Catalog Number :

45602

Product Name :

pCRII-Topo Diap3 in situ probe

article :

doi	10.1016/j.neuron.2004.12.036
id	6751
pubmed_id	15664173

bacterial resistance :

Ampicillin

cloning :

backbone

pCRII-Topo

backbone_mutation

backbone_origin

Invitrogen

backbone_size

4000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

in situ

growth notes :

Fragment was amplified using the following primers: AGCAGTTCGCTGTTGTGATG GCACGTTTCTCCTTTTCTGC For in vitro transcription, use the SpeI restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and SP6 promoter for antisense probe generation. Please note that Addgene's sequencing results match bp# 1589-2321 of BC028920, not bp# 1610-2321 as indicated on plasmid map. The plasmid functions as described in the associated publication.

origin :

pi :

alt_names

Diap3

diaphanous homolog 3 (Drosophila)

cloning

clone_method	TOPO Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	T7, M13 Forward
sequencing_primer_5	M13 Rev, Sp6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	4930417P13Rik, Dia2, Diap3, Drf3, p134MDia2
gene	Diaph3
id	56419

genbank_ids

BC028920

mutation

fragment contains bp# 1589-2321 of BC028920

name

Diap3 in situ probe

shRNA_sequence

size

695

species

10090

Mus musculus