This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-flex-taCasp3-TEVp
catalog :
45580
citations: 29
Reference
Narimatsu Y, Kato M, Iwakoshi Ukena E, Moriwaki S, Ogasawara A, Furumitsu M, et al. Neurosecretory Protein GM-Expressing Neurons Participate in Lipid Storage and Inflammation in Newly Developed Cre Driver Male Mice. Biomedicines. 2023;11: pubmed publisher
Mata Pacheco V, Hernandez J, Varma N, Xu J, Sayers S, Le N, et al. Dynamic, sex- and diet-specific pleiotropism in the PAC1 receptor-mediated regulation of arcuate proopiomelanocortin and Neuropeptide Y/Agouti related peptide neuronal excitability by anorexigenic ventromedial nucleus PACAP neurons. J Neuroendocrinol. 2023;:e13357 pubmed publisher
Go xf1 i Erro H, Selvan R, Leiras R, Kiehn O. Pedunculopontine Chx10+ neurons control global motor arrest in mice. Nat Neurosci. 2023;: pubmed publisher
Schroer J, Warm D, De Rosa F, Luhmann H, Sinning A. Activity-dependent regulation of the BAX/BCL-2 pathway protects cortical neurons from apoptotic death during early development. Cell Mol Life Sci. 2023;80:175 pubmed publisher
de Araújo A, Braga I, Leme G, Singh A, McDougle M, Smith J, et al. Asymmetric control of food intake by left and right vagal sensory neurons. bioRxiv. 2023;: pubmed publisher
Inaba H, Li H, Kawatake Kuno A, Dewa K, Nagai J, Oishi N, et al. GPCR-mediated calcium and cAMP signaling determines psychosocial stress susceptibility and resiliency. Sci Adv. 2023;9:eade5397 pubmed publisher
Sandoval Rodr xed guez R, Parra Reyes J, Han W, Rueda Orozco P, Perez I, de Araujo I, et al. D1 and D2 neurons in the nucleus accumbens enable positive and negative control over sugar intake in mice. Cell Rep. 2023;42:112190 pubmed publisher
Qian Y, Li J, Zhao S, Matthews E, Adoff M, Zhong W, et al. Programmable RNA sensing for cell monitoring and manipulation. Nature. 2022;610:713-721 pubmed publisher
Zhang T, Perkins M, Chang H, Han W, de Araujo I. An inter-organ neural circuit for appetite suppression. Cell. 2022;185:2478-2494.e28 pubmed publisher
Tsai N, Wang F, Toma K, Yin C, Takatoh J, Pai E, et al. Trans-Seq maps a selective mammalian retinotectal synapse instructed by Nephronectin. Nat Neurosci. 2022;25:659-674 pubmed publisher
Topilko T, Diaz S, Pacheco C, Verny F, Rousseau C, Kirst C, et al. Edinger-Westphal peptidergic neurons enable maternal preparatory nesting. Neuron. 2022;110:1385-1399.e8 pubmed publisher
Siemian J, Arenivar M, Sarsfield S, Borja C, Russell C, Aponte Y. Lateral hypothalamic LEPR neurons drive appetitive but not consummatory behaviors. Cell Rep. 2021;36:109615 pubmed publisher
Zhang Z, Reis F, He Y, Park J, DiVittorio J, Sivakumar N, et al. Estrogen-sensitive medial preoptic area neurons coordinate torpor in mice. Nat Commun. 2020;11:6378 pubmed publisher
Ruediger S, Scanziani M. Learning speed and detection sensitivity controlled by distinct cortico-fugal neurons in visual cortex. elife. 2020;9: pubmed publisher
Usseglio G, Gatier E, Heuzé A, Hérent C, Bouvier J. Control of Orienting Movements and Locomotion by Projection-Defined Subsets of Brainstem V2a Neurons. Curr Biol. 2020;30:4665-4681.e6 pubmed publisher
Yu X, Ba W, Zhao G, Ma Y, Harding E, Yin L, et al. Dysfunction of ventral tegmental area GABA neurons causes mania-like behavior. Mol Psychiatry. 2020;: pubmed publisher
Mazuski C, Chen S, Herzog E. Different Roles for VIP Neurons in the Neonatal and Adult Suprachiasmatic Nucleus. J Biol Rhythms. 2020;:748730420932073 pubmed publisher
Chiang M, Nguyen E, Canto Bustos M, Papale A, Oswald A, Ross S. Divergent Neural Pathways Emanating from the Lateral Parabrachial Nucleus Mediate Distinct Components of the Pain Response. Neuron. 2020;106:927-939.e5 pubmed publisher
Inoue S, Yang R, Tantry A, Davis C, Yang T, Knoedler J, et al. Periodic Remodeling in a Neural Circuit Governs Timing of Female Sexual Behavior. Cell. 2019;179:1393-1408.e16 pubmed publisher
Li H, Vento P, Parrilla Carrero J, Pullmann D, Chao Y, Eid M, et al. Three Rostromedial Tegmental Afferents Drive Triply Dissociable Aspects of Punishment Learning and Aversive Valence Encoding. Neuron. 2019;104:987-999.e4 pubmed publisher
Smith M, Choudhury A, Glegola J, Viskaitis P, Irvine E, de Campos Silva P, et al. Extrahypothalamic GABAergic nociceptin-expressing neurons regulate AgRP neuron activity to control feeding behavior. J Clin Invest. 2020;130:126-142 pubmed publisher
Wu J, Kung J, Dong J, Chang L, Xie C, Habib A, et al. Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning. Cell Rep. 2019;28:1167-1181.e7 pubmed publisher
Li M, Madara J, Steger J, Krashes M, Balthasar N, Campbell J, et al. The Paraventricular Hypothalamus Regulates Satiety and Prevents Obesity via Two Genetically Distinct Circuits. Neuron. 2019;102:653-667.e6 pubmed publisher
Bayless D, Yang T, Mason M, Susanto A, Lobdell A, Shah N. Limbic Neurons Shape Sex Recognition and Social Behavior in Sexually Naive Males. Cell. 2019;176:1190-1205.e20 pubmed publisher
Zhang G, Shen L, Zhong W, Xiong Y, Zhang L, Tao H. Transforming Sensory Cues into Aversive Emotion via Septal-Habenular Pathway. Neuron. 2018;99:1016-1028.e5 pubmed publisher
Resch J, Fenselau H, Madara J, Wu C, Campbell J, Lyubetskaya A, et al. Aldosterone-Sensing Neurons in the NTS Exhibit State-Dependent Pacemaker Activity and Drive Sodium Appetite via Synergy with Angiotensin II Signaling. Neuron. 2017;96:190-206.e7 pubmed publisher
Riera C, Tsaousidou E, Halloran J, Follett P, Hahn O, Pereira M, et al. The Sense of Smell Impacts Metabolic Health and Obesity. Cell Metab. 2017;26:198-211.e5 pubmed publisher
Zhao Z, Wang L, Gao W, Hu F, Zhang J, Ren Y, et al. A Central Catecholaminergic Circuit Controls Blood Glucose Levels during Stress. Neuron. 2017;95:138-152.e5 pubmed publisher
Yang C, Chiang M, Gray D, Prabhakaran M, Alvarado M, Juntti S, et al. Sexually dimorphic neurons in the ventromedial hypothalamus govern mating in both sexes and aggression in males. Cell. 2013;153:896-909 pubmed publisher
product information
Catalog Number :
45580
Product Name :
pAAV-flex-taCasp3-TEVp
article :
doi10.1016/j.cell.2013.04.017
id6799
pubmed_id23663785
bacterial resistance :
Ampicillin
cloning :
backbonepAAV-MCS
backbone_mutation
backbone_originStratagene
backbone_size5347
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
AAV
Other
Adeno Associated Viral Vector
growth notes :
The taCasp3-T2A-TEVp transgene was generated by overlapping PCR of plasmids harboring taCasp3 and TEVp (Gray et al., 2010; Cell 142, 637-646 PMID: 20723762). This transgene was inserted in reverse orientation into the plasmid pAAV-EF1a-DIO-hChR2(H134R)-EYFP-WPRE-pA such that it replaced hChR2(H134R)-EYFP
growth temp :
For liquid cultures grow in LB:2XYT (1:1) overnight at 30 C.
origin :
30
pi :
alt_names
Casp3
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterEF1a
sequencing_primer_3AAT CAT GTC CCT GCC GTC GAT C
sequencing_primer_5AAA GCA GCG TAT CCA C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCPP32, CPP32B, SCA-1
geneCASP3
id836
genbank_ids
mutationLinker replaced with a TEV protease cleavage site
nameCaspase 3
shRNA_sequence
size847
species
9606
Homo sapiens
100
Synthetic
tags
alt_names
TEV protease
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterEF1a
sequencing_primer_3AGA GGG GAT CGT TGT AGA AGT C
sequencing_primer_5TTC TCA AGC CTC AGA CAG TGG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameTobacco Etch Virus protease
shRNA_sequence
size705
species
tags
resistance markers :
1567
1632
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA