pCI-EGFP-NR2b wt | Addgene 45447 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCI-EGFP-NR2b wt

catalog :

45447

citations: 5

Reference
Angelini C, Morellato A, Alfieri A, Pavinato L, Cravero T, Bianciotto O, et al. p140Cap regulates the composition and localization of the NMDAR complex in synaptic lipid rafts. J Neurosci. 2022;42:7183-200 pubmed publisher
Vasavda C, Semenza E, Liew J, Kothari R, Dhindsa R, Shanmukha S, et al. Biliverdin reductase bridges focal adhesion kinase to Src to modulate synaptic signaling. Sci Signal. 2022;15:eabh3066 pubmed publisher
Ortiz Sanz C, Balantzategi U, Quintela López T, Ruiz A, Luchena C, Zuazo Ibarra J, et al. Amyloid β / PKC-dependent alterations in NMDA receptor composition are detected in early stages of Alzheimer´s disease. Cell Death Dis. 2022;13:253 pubmed publisher
Bai X, Karasmanis E, Spiliotis E. Septin 9 interacts with kinesin KIF17 and interferes with the mechanism of NMDA receptor cargo binding and transport. Mol Biol Cell. 2016;27:897-906 pubmed publisher
Barria A, Malinow R. Subunit-specific NMDA receptor trafficking to synapses. Neuron. 2002;35:345-53 pubmed

product information

Catalog Number :

45447

Product Name :

pCI-EGFP-NR2b wt

article :

doi	10.1016/S0896-6273(02)00776-6
id	6702
pubmed_id	12160751

bacterial resistance :

Ampicillin

cloning :

backbone

pCI

backbone_mutation

backbone_origin

Promega

backbone_size

4006

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Addgene Next-generation sequencing identified a sequence discrepancy that results in a Met to Leu change at position 745 in the plasmid insert, relative to Genbank IDs NM_012574.1 and NP_036706.1. The plasmid is expected to function as described in the associated publication.

growth temp :

Note: This plasmid causes extremely slow growth. The depositing lab notes that growth times longer than the standard growth period may be required.

origin :

pi :

alt_names

NMDAR2B

Grin2b

GluN2B

cloning

clone_method	Restriction Enzyme
cloning_site_3	KpnI
cloning_site_5	NheI
promoter	CMV
sequencing_primer_3	EBV-rev
sequencing_primer_5	T7; chim-int-F (TCTTACTGACATCCACTTTGCC)
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	GluN2B
gene	Grin2b
id	24410

genbank_ids

mutation

EGFP inserted after the predicted signal peptide cleavage site (after amino acid residue 31)

name

NR2B

shRNA_sequence

size

5700

species

10116

Rattus norvegicus